Testing some extracts of plants from the Danube Delta with potential antiparasitic effect on equines
Journal Title: Scientific Annals of the Danube Delta Institute - Year 2015, Vol 21, Issue
Abstract
The study was done during november 2016 on strongyls eggs from feces from a total of 28 horses in Tulcea county, C.A. Rosetti locality in the Danube Delta. To determine the degree of infestation was used the method McMaster and Stoll (adapted for quantitative larvohelmintology). The tests of hatching eggs (egg hatching assay - EHA) and larval development (larvae development assay - LDA) were made, using as active substances herbal decoctions and hydro-alcoholic solutions made from well known anthelmintic plants. One decoction and two hydro-alcoholic extracts were tested, Hippophae rhamnoides (sea buckthorn) respectively Thymus serpyllum (creeping thyme) and Arthemisia absinthium (wormwood). In order to achieve the two tests for therapeutic efficacy of the tested products were obtained six serial dilutions with concentrations of 50%, 25%, 12.5%, 6.25%, 3.12%, and 1, 56%. For decoctions were made control samples with distilled water, and with alcohol for hydroalcoholic solutions. The data obtained in both tests were analyzed using Anthelmintic Rezistance Program (ARP), determining the parameters "a" and "b" to trace the line prediction and lethal concentrations (CL). We can be concluded that for the decoction was observed an emphatic decrease of the percentage of larval development. For the two hydro-alcoholic extracts the hatching percentage of Strongyls eggs was different depending on the concentration used, the maximum value being registered at 25% dilution, in which the percentage of hatching was 82.35%, and the minimum concentration of 12.50%. LDA test showed a significant reduction in the percentage of larval Strongyls development, stage 3, at all dilutions, which is less than 50%.
Authors and Affiliations
Stefan Raileanu, Mihai Cernea, Violeta Tamasu
Keywords
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- EP ID EP235217
- DOI 10.7427/DDI.21.08
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