The Ameliorative Effect of Vitamin a and Stem Enhancer on Cytological Changes and Apoptosis Inducing-Activity in BPA-Treated Murine Model
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2018, Vol 6, Issue 1
Abstract
Bisphenol A is a plasticizer used as monomer of epoxy resins and polycarbonate plastics which found in many different applications such as adhesives and paper coatings, food related containers and water bottles; what make human is more exposed to its hazard effects especially at specific doses. The present study was performed with the purpose of evaluating the ameliorating property of stem enhance and vitamin A against BPA toxicity on liver and kidney tissues. In this experimental study, 20 female albino rats were divided into 4 groups. Rats of BPA group was administered intraperitoneally daily dose of BPA (20mg/kg bw) for 45 days; moreover oral administration of vitamin A and stemenhance was used as ameliorative agents for other 15 successive days after BPA treatment period. At the end , rats were sacrificed and their liver and kidney were proceeded for histopathological and immune-histochemical reactivity (caspase 3). Agarose gel electrophoresis was carried out to evaluate the pattern of DNA damage furthermore, morphological examination of hepatocyte was carried out by acridine orange/ ethidium bromide (AO/EB) dual fluorescent staining. BPA caused inflammation, apoptosis and vacuolization in liver and kidney. Also caspase-3 immunohistochemical reactions showed strong intensity in BPA group in both liver and kidney tissues. While in the treated groups with stemenhance and vitamin A showed amelioration in DNA fragmentation, histopathological changes and intensity of caspase-3 immunoreactivity. Vitamin A has ameliorative effect more than stemenhance on liver and kidney against BPA supplying injurious molecular effects. Vitamin A and stemenhance can be considered as a natural supplements against environmental toxicity.
Authors and Affiliations
Hassan BN, Al beltagy RS, AL Azzouni AS, El-Garawani IM
Keywords
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