The Ameliorative Effects of Pycnogenol® on Liver Ischemia-Reperfusion Injury in Rats
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2017, Vol 14, Issue 3
Abstract
Objectives: Pycnogenol® (PYC®), a standardized extract from the bark of Pinus maritima, consists of different phenolic compounds. PYC® has shown to have protective effects on chronic diseases such as diabetes, asthma, cancer, and immune disorders. The aim of this study was to determine the effects of PYC® against the DNA damage and biochemical changes in blood, liver, and lung tissues of ischemia-reperfusion (IR)-induced Wistar albino rats. Materials and Methods: A sham group, IR injury-induced group, and IR+PYC® group were formed. Ischemia was induced and sustained for 45 min, then the ischemic liver was reperfused, which was sustained for a further 120 min at the end of this period. After anesthesia and before the IR inducement, 100 mg/kg PYC® was given to the IR+PYC® group through intraperitoneal injections. The total oxidant (TOS) and total antioxidant status (TAS), total thiol levels (TTL), advanced oxidation protein products (AOPP), and biochemical parameters [myeloperoxidase (MPO), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH)] in the rats were analyzed using spectrophotometric methods and DNA damage was assessed using single-cell gel electrophoresis. Results: The levels of TOS, TTL, MPO, AOPP, ALT, AST, and LDH were significantly decreased in the IR+PYC® group compared with the IR group (p<0.05). The levels of TAS were significantly increased in the IR+PYC® group compared with the IR group (p<0.05). PYC® reduced the DNA damage when compared with the IR group (p<0.05). Conclusion: The present results suggest that PYC® treatment might have a role in the prevention of IR-induced oxidative damage by decreasing DNA damage and increasing antioxidant status.
Authors and Affiliations
Mehmet TOKAÇ, Merve BACANLI, Ersin Gürkan DUMLU, Sevtap AYDIN, Birkan BOZKURT, Abdüssamed YALÇIN, Özcan EREL, Nurşen BAŞARAN
Keywords
Related Articles
- EP ID EP381005
- DOI 10.4274/tjps.49369
- Views 132
- Downloads 0
Superior Solubility and Dissolution of Zaltoprofen via Pharmaceutical Cocrystals
Objectives: Pharmaceutical cocrystals are a promising tool to enhance the solubility and dissolution of poorly soluble drugs. Zaltoprofen (ZFN) is nonsteroidal anti-inflammatory drug with a prevalent solubility problem....
Development of a Discriminative and Biorelevant Dissolution Test Method for Atorvastatin/Fenofibrate Combination with Appliance of Derivative Spectrophotometry
Objectives: Nowadays, the market is flooded with combinations of drugs in various dosage forms, but there is a lack of official methods to quantify them. A single dissolution test method for the analysis of combined dosa...
Characterization of Gamma-Irradiated Rosmarinus officinalis L. (Rosemary)
Objectives: Rosmarinus officinalis L., a member of the family Lamiaceae, is regarded as the spice with the highest antioxidant activity. Materials and Methods: In this study, the transmission electron microscopy, X-ray d...
Quantification of Tamsulosin Hydrochloride and Solifenacin Succinate by Discriminative Derivative Synchronous Emission Spectroscopy
Objectives: The present study was undertaken with the objective to develop and validate a simple spectrofluorimetric method for the simultaneous quantification of tamsulosin hydrochloride and solifenacin succinate. Mate...
Development of Andrographolide-Carboxymethyl Chitosan Nanoparticles: Characterization, in vitro Release and in vivo Antimalarial Activity Study
Objectives: The purpose of this study was to investigate the effect of andrographolide-carboxymethyl chitosan nanoparticles formation on the physical characteristics, in vitro release profile and in vivo antimalarial act...