The analysis of cytochrome b nucleotidic sequence for Carassius gibelio (Bloch, 1782)
Journal Title: Aquaculture, Aquarium, Conservation & Legislation - International Journal of the Bioflux Society - Year 2009, Vol 2, Issue 1
Abstract
The paper is part of a larger scale study for some genes` (Cytb, ND4L and D-loop) nucleotidic structure identification by sequencing, to distinguish the structural differences and their exact length in base pairs. Research was carried out on individuals of Carassius gibelio (Bloch, 1782) (Actinopterygii, Cypriniformes) from two different populations, Iezăreni and Movileni (Iași), from which dorsal muscular tissue was sampled. Mitochondrial DNA (mtDNA) isolation and purification was carried out automatically using Promega’s Maxwell 16 (SEV module). Cytochrome b (cytb) was multiplied by a two stage polymerase chain reaction (PCR), using two sets of complementary primers (1 set for each fragment). Direct sequencing of PCR products revealed that the cytochrome b has one sequence of 1140bp. The obtained sequences were subsequently compared with sequences of the same gene from other individuals within this species, towards identifying possible differences in the nucleotidic structure.
Authors and Affiliations
Lucian Gorgan
Keywords
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