The Analysis of Genetic Polymorphism. The Relationship between Interleukin – 4 Polymorphisms and Intraepithelial Cervical Neoplasia
Journal Title: Applied Medical Informatics - Year 2010, Vol 27, Issue 3
Abstract
[i]Objectives[/i]: Interleukin 4 plays a critical role in T helper 2 responses to HPV infection and angiogenesis. The present study aim to study the association between the IL4 promoter polymorphism – 590 C>T, respectively VNTR intron 2 polymorphism and cervical intraepithelial neoplasia. [i]Material and Method[/i]: We have realized a prospective case controls study that included 128 cases of intraepithelial neoplasia positive for HPV HR testing and 111 controls negative for intraepithelial lesion and also negative for HPV HR. Clinical examination was performed on each patient; blood and cervical sample were obtained. Cervical probes were analyzed regarding cytology and HPV HR testing. From peripheral blood DNA sample was obtain followed by genotype analysis for IL4 -590 C>T using PCR RFLP, respectively IL4 70 bp VNTR determined by PCR. Results: The absolute frequency of genotypes for IL4 -590 C>T was T/T-5, C/T-42, C/C-81 in the cases group respectively T/T-2, C/T-32, C/C-77 in the control group. The chi-square test had a value of 0.983 (p=0.321) while considering the presence of a minimum one single variant allele as a risk factor for cervical cancer, respectively 0.926 (p=0.336) for homozygous variant genotype. Odds ratio was 0.761 (95%CI [0.443-1.306]) while considering C/T+T/T respectively 2R/3R, 2R/2R as a risk factor, and 0.451 (95%CI 95% [0.086-2.374]) - TT respectively 2R/2R as a risk factor. [i]Conclusion[/i]: No linear statistical significant association has been found between IL4 polymorphism and cervical neoplasia (p = 0.322).
Authors and Affiliations
Ioana ROTAR, Sorana BOLBOACĂ, Daniel MUREŞAN, Radu POPP, Marius FĂRCAŞ, Felicia PETRIŞOR, Cristina BUTUZA, Gabriela CARACOSTEA, Florin STAMATIAN
Keywords
Related Articles
- EP ID EP113364
- DOI -
- Views 123
- Downloads 0
Obesity as a Risk Factor for Biliary Lithiasis - Clinical Study
The purpose of this study is to bring new data regarding the prevalence of biliary lithiasis in our region (Cluj district) and regarding the role of obesity as a risk factor for this pathology. We performed a clinical st...
The identification of the level of knowledge of nurses in the field of hand hygiene through a questionnaire applied within an educational program
Introduction: The level of knowledge on hand hygiene (HH) and healthcare associated infections (HAI) directly influences the practice of HH of medical staff and the rate of HAI. Continuous Medical Education (EMC) is an i...
Using Electronic Health Record Systems in Medical Education – A Needs Assessment
The aim of this study was to determine the features needed in electronic health record systems by supervising physicians for teaching medical students and residents. We conducted in-depth semi-structured interviews with...
Does the Use of Ovulation Monitors Really Increase Pregnancy Rates? Some Things Women Should Know
Ovulation monitors are widely used by women wishing to achieve pregnancy. However, there are few data substantiating claims that these devices enhance the probability of becoming pregnant. In one report it is concluded f...
Data Sources and Information to be Collected for a Romanian Diabetes Register
Diabetes is a serious condition that damages many systems and organs, mainly the blood vessels and the nerves. The importance of the diabetes and its complications, along with the prevalence of the disease makes it a maj...