The anti-planktonic and anti-biofilm formation activity of Iranian pomegranate peel hydro-extract against Staphylococcus aureus
Journal Title: Iranian Journal of Veterinary Science and Technology (IJVST) - Year 2020, Vol 12, Issue 1
Abstract
Staphylococcal infections and contaminations have elicited a growing and perennial concern in the medical and food industries. Meanwhile, the manifestation of antibiotic-resistant strains such as methicillin-resistant Staphylococcus aureus (MRSA) beside the production of disinfectant-resistant biofilms makes the confrontation with the bacteria more cumbersome and challenging. Pomegranate peel as a waste product of juicing factories is a natural antibacterial agent. The pomegranate peel hydro-extract (PPHE), as a bio-friendly material, was prepared from an Iranian pomegranate cultivar, Rabab, and its phenolic compounds and antioxidant (via DPPH and FRAP assays) and anti-staphylococcal (anti-planktonic and anti-biofilm) properties were assessed. The Rabab PPHE inhibited planktonic cells and biofilm formation of three S. aureus. The Rabab PPHE produced large and obvious staphylococcal inhibition zones in which their diameters were significantly dose-dependent for the milk isolated S. aureus (p < 0.05). Despite the resistance of MRSA (ATCC 33591) to beta-lactam antibiotics, the minimum inhibitory concentration (MIC) of PPHE against its planktonic cells was only 3.75mg mL-1. Furthermore, Rabab PPHE inhibited bacterial biofilms formation in a dose-dependent manner. The MIC of Rabab PPHE against planktonic milk-isolated S. aureus, S. aureus (ATCC 29737), and MRSA prevented 47, 36, and 26% of their biofilm formation, respectively. This addresses the differences between the anti-planktonic and anti-biofilm activity of Rabab PPHE. The anti-planktonic and to a lesser extent the anti-biofilm forming activity of this water-based extract supports the notion of its effectiveness and salubrious application in food and pharmaceutical industries.
Authors and Affiliations
Hadi Ebrahimnejad,Maryam Ebadi,Ladan Mansouri-Najand,
Keywords
Related Articles
- EP ID EP722390
- DOI -
- Views 33
- Downloads 1
Effects of Oral Exposure to Titanium Dioxide Nanoparticles on the Liver, Small Intestine, and Kidney of Rats assessed by light microscopy and Transmission Electron Microscopy
The TiO2 NPs are widely used in many commercial products, nanomedicine, agriculture, personal care products, different industries, and pharmaceutical preparations with potential risks to human health and the environment....
A concise review on the anatomy of the atrioventricular node in mammals
Cardiac conduction system enables the heart to pulsate continuously by producing electric impulse and conducting it. As the anatomical information about cardiac conduction system in human being and other mammals needed i...
Isolation and culturing myogenic satellite cells from ovine skeletal muscle
Sheep satellite cells more than satellite cells of the rat and mouse are similar to human satellite cells. These cells are widely used in the modeling and treatment of diseases like heart insufficiency, neurological dise...
Identification of bovine ephemeral fever (BEF) outbreak in a large dairy farm in Varamin, Iran
Bovine Ephemeral Fever (BEF) flared up in a dairy farm with 2097 animals. The disease started in September, 2006, with daily means of environmental temperature (ET) and relative humidity (RH) of 23.8 ºC and 37%, respect...
Evaluation of histopathological features of Argas reflexus bite in pigeon
Argas reflexus is considered obligate blood feeder that may cause skin redness and local swelling. For histopathological evaluation of biting site, pigeons were used as natural host species in this study. Adult ticks wer...