The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines
Journal Title: Turkish Journal of Pharmaceutical Sciences - Year 2019, Vol 16, Issue 3
Abstract
Objectives: Natural products originating from plants have been used for many years in the treatment of various diseases, including cancer. Centaurea solstitialis subsp. solstitialis is used in Turkish folk medicine. This study was the first to determine the in vitro biological effects of ethanolic extract from the flowering parts of C. solstitialis L. subsp. solstitialis collected from Muğla Province. Materials and Methods: The cytotoxic effect was evaluated against Daudi, A549, and HeLa cancer cells and one normal BEAS-2B cell line using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- dipenyltetrazolium bromide) assay. Flow cytometric analysis and the caspase-3 activity assay were performed to detect apoptotic cell death. Angiogenic factor [vascular endothelial growth factor (VEGF)] secretion and the release of interleukin (IL)-1α, IL-6, and tumor necrosis factor (TNF)-α by cells treated with the extract were measured using enzyme-linked immunosorbent assay. Results: The extract exhibited cytotoxic effects against all the cancer cell lines used but HeLa and Daudi were the most sensitive cells, with IC50 values of 63.18 μg/mL and 69.27 μg/mL, respectively. Selective cytotoxicity was observed between the HeLa and normal BEAS-2B cell lines. The extract arrested the cell cycle at the S and G2 phases. In addition, apoptotic cell death was detected in HeLa and A549 cells. Moreover, the plant extract caused a significant decrease in VEGF secretion in A549 cells and a fluctuation in IL-1α, IL-6, and TNF-α secretion in A549 and Daudi cells. Conclusion: These observations suggest that the flowering parts of C. solstitialis may be a potential source in the development of natural drugs for the treatment of cancer and modulation of cytokine secretion.
Authors and Affiliations
Mehlika ALPER, Hatice GÜNEŞ
Keywords
Related Articles
- EP ID EP604398
- DOI 10.4274/tjps.galenos.2018.27146
- Views 114
- Downloads 0
An Overview of iQOS® as a New Heat-Not-Burn Tobacco Product and Its Potential Effects on Human Health and the Environment
Tobacco smoke from regular cigarettes contains a number of harmful chemicals such as nicotine, arsenic, benzene, carbon monoxide, heavy metals, and tobacco-derived nitrosamines. About 1% of over 7000 chemical substances...
Therapeutic Protection from Hepatic Injury and Chemical Constituents of Buchanania angustifolia Roxb
Objectives: The use of modern medicines in the treatment of diseases instead of traditional medicine is causing different adverse effects. Therefore, there is need to search new bioactive compounds to treat different dis...
The Apoptotic and Anti-apoptotic Effects of Pendimethalin and Trifluralin on A549 Cells In Vitro
Objectives: Pendimethalin and trifluralin are commonly used in many countries to control broadleaf weeds and grassy weed species because of their inhibitor effects on growth and cell division. In this study, we examined...
Desloratadine-Eudragit® RS100 Nanoparticles: Formulation and Characterization
Objectives: The objective of the present study was to formulate Desloratadine-Eudragit® RS100 nanoparticles and investigate the characteristics of the prepared nanoparticles. Materials and Methods: The nanoparticles wer...
Anatomic Studies on Verbascum pestalozzae Boiss. and Verbascum pycnostachyum Boiss. & Heldr.
Objectives: The genus Verbascum L. (Scrophulariaceae) known as “sığır kuyruğu” in Anatolia is represented by 248 species, 193 of which are endemic. The flowers contain essential oil, mucilage, and glycosides. Some specie...