The applicability of Beckman PK7300 fully automatic blood group instrument for TPPA detection
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 1
Abstract
Objective To investigate the applicability of Beckman PK7300 for TPPA testing on anti-TP reactive specimens from blood donors. Methods 1) The TPPA test using Beckman PK7300 (abbreviated as instrument method) had been established and the performance was verificated by calculating the total compliance rate, positive compliance rate and negative compliance rate as compared with the manual method. The repeatability of this instrument method was also evaluated. 2) The applicability of the instrument method was evaluated by examing 555 TP-reactive samples for 6 consecutive days, so as to analyze the readable reading rate, agglutination strength distribution and other control methods. Results 1) The total, positive, and negative compliance rates of TPPA detection by both instrumental and manual methods were 100% (kappa value =1). The SPC value of samples, read manually as " + + ", was less than or equal to 3 by Beckman PK7300; the SPC value of samples, read manually as " -", was greater than or equal to 20 by Beckman PK7300. The two methods were well consistent. The instrument method was repeated for 12 times for the same samples, and the accuracy rate was 100% (12 / 12), with good repeatability.2) The results of the TPPA test in 555 anti-TP reactivity specimens showed an overall readable rate of 99.82%(554/555). The SPC values of the negative and unsensitized particles of TPPA were distributed on both sides of the determination value without crossover. The control and monitor thoughout the test were carried out automatically by the instrument. Conclusion The TPPA test conducted by the Beckman PK7300 fully automatic blood group instrument is suitable for the confirmatory experiment of anti-TP reactive specimens in blood center laboratories, which could realize the automation and standardization of TPPA detection.
Authors and Affiliations
Junjie JIA, Zhengmin LIU, Rui WANG, Nan GAO, Rui LIU, Jin GUO
Keywords
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- EP ID EP738469
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.01.018
- Views 6
- Downloads 0
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