The Application Of PCR RAPD Technique In Determining Female Sex Of Kapulasan (Nepheleum Lapaceum Mutabile)
Journal Title: JERAMI Indonesian Journal of Crop Science - Year 2019, Vol 1, Issue 2
Abstract
Kapulasan is one of horticultural plants derived from tropical region that has significant economic value. One of the obstacles in cultivating the plant is that kapulasan has flowering type of androdioceus, i.e. the male and the female flowers are separated. Female flowers produce hermaphroditic flowers that can produce fruits while the male ones will not produce fruits. In the cultivation of the plant, it is very difficult to determine the female flowers if they are propagated by seeds. It takes up to 7 years to determine the nature of the flowering. One of the important steps that can be done is through a system of early detection using molecular marks. RAPD technique as a simple technique can be used to distinguish the characteristics. The results of the research show that of the 250 primers used, the OPM-09 primer produces female sex bands related to the controller of the female kapulasan plants of which the ribbon produced is 512 bp in size.
Authors and Affiliations
Edi Wirman
Effect of Different Seed Water Content and Storage Duration towards Seed Viability of Local Genotype Brown Rice Daro Merah
Most of rice seeds experience after ripening problem which then result in seed dormancy. This kind of dormancy could be broken through various types of treatments, including storaging in dry place for certain period. Thi...
Micropropagationof Male and Female Trees of Andaleh (MorusmacrouraMiq.) through In-vitro Culture using Several Compositionsof Basal Medium
Andalehis the local name of MorusmacrouraMiq. in West Sumatra, Indonesia. Nowadays, this dioeciously speciesis in endangered situation. The aim of the research is to find out the appropriate combination of plant gr...
The Effect of Shallot Bulbs Storage Duration and Paclobutrazol Treatments to Disease Attacks on Shallot Plant in Karo Highlands
A major challenge in the development of shallot seeds is the limited availability of qualified seed due to pathogen infection during the vegetative phase. The study aims to determine the effect of storage duration and pa...
The Application Of PCR RAPD Technique In Determining Female Sex Of Kapulasan (Nepheleum Lapaceum Mutabile)
Kapulasan is one of horticultural plants derived from tropical region that has significant economic value. One of the obstacles in cultivating the plant is that kapulasan has flowering type of androdioceus, i.e. the male...
Genetic Diversity Of Age, Plant Height And Number Of Grain Per Panicle Characters Of F3 Generation Derived From Crossing Silopuk With Fatmawati Varieties
This study aims to determine the population and genetic parameters of population F3 result of selection pedigri red rice crosses Silopuk with Fatmawati Superior Varieties using Augmented Design. Silopuk cultivars with re...