The application of the RAPD-PCR method for identification of lactic acid bacteria
Journal Title: Біологія тварин - Year 2014, Vol 16, Issue 4
Abstract
The heterogeneous properties of lactic acid bacteria (LAB) create a significant difficulties in their identification. The use of genotypic methods based on polymerase chain reaction (PCR) has opened new possibilities for precise and rapid identification of LAB. Our purpose was to identify LAB isolated from ewe’s cheese that is traditionally produced in the Carpathian region of Ukraine. A number of microbiological and molecular genetic studies, such as cultural and morphological properties of the isolates LAB and the method of analysis random amplification of polymorphic DNA (RAPD-PCR) have been used to achieve this purpose. The material for researches was ewe’s cheese buts (brine cheese before ripening) produced in the alpine mountain valley of Putyla district of Chernivtsi region. A pure cultures LAB, which were isolated from the cheese, served as subject of the research. The reference strains of LAB we used for the comparative analysis of amplified fragments of DNA of the investigated strains of LAB. The investigated strains of LAB were taken to family Lactobacteriaceae and Streptococcaceae under the nature of growth of colonies and the temperature conditions of cultivating and to the genus Lactobacterium, Lactococcus and Streptococcus under the morphological properties of isolates. The considerable differences in electrophoretic movable were found between the investigated strains of LAB that consisted in the absence of similar, general fragments of DNA, and also a small number of identical DNA fragments were found in some spectra. The absence of an identical DNA profiles indicates heterogeneity properties of isolated LAB and their intraspecific diversity. RAPD-PCR method is appropriate to identify generic affinity isolates of LAB detection and intraspecific changes but not sufficient for species and strains identification of LAB.
Authors and Affiliations
I. M. Slyvka, O. Y. Tsisaryk, T. Bocer
Keywords
Related Articles
- EP ID EP355928
- DOI -
- Views 78
- Downloads 0
Epizootic situation on animal rabies in the Northern and Western regions of Ukraine
One of the most significant infectious diseases of animals in the territory of Ukraine is rabies. In Ukraine, a significant number of outbreaks of this disease are recorded among animals of different species, namely wild...
Molecular and genetic characteristics isolates virus of porcine enzootic encephalomyelitis isolated from wild boar and domestic swine on the territory of Ukraine
The article provide data about detection two isolates viruses of porcine enzootic encephalomyelitis from the territory of Ukraine isolated from domestic piglet and wild boar. Porcine enzootic encephalomyelitis virus isol...
The influence of high nervous activity type on superoxide dismutase activity and content of Copper and Zinc in the blood of cows
New scientific data concerning the degree and nature of the influence of the main characteristics of cortical processes on the activity of superoxide dismutase and the content of Copper and Zinc in the blood of cows are...
Acute toxicity of the mospilan for laboratory animals
Neonicotinoids are the insecticides of systemic and contact-intestinal action with translaminar activity. In agriculture it is applied against sucking, leaf-eating insects and soil pests, treatments for vegetative crops...
The resistance of rabbit organism for the effect of sulfur complex
The article presents the results of the study of the influence of the production of rabbits from different periods of 60 to 118 days of various amounts of sulfur citrate, obtained by the method using nanotechnology and s...