The Characterization of Bacterial Cellulose Produced by Acetobacter xylinum and Komgataeibacter saccharovorans under Optimized Fermentation Conditions
Journal Title: Microbiology Research Journal International - Year 2015, Vol 9, Issue 3
Abstract
Aims: Studying the biological activities of A. xylinum ATCC 10245 (as reference strain) and K. saccharivorans PE5 for bacterial cellulose production (B.C) under optimized fermentation conditions and the structural characteristics of cellulose produced. Study Design: The production of bacterial cellulose under optimal fermentation conditions by investigated strains then the purified cellulose was characterized by different techniques. Place and Duration of Study: Agric. Microbiology Dept., Fac. of Agriculture, Ain Shams Univ., Cairo, Egypt. Methodology: A. xylinum ATCC 10245 and K. saccharivorans PE5 strain were used as producers of bacterial cellulose. These strain were grown on productive medium under optimal fermentation conditions. The parameters of growth and B.C production were determined during the fermentation period. The specific growth rate, doubling time. Multiplication rate and generation number were calculated during the exponential phase of growth. Purified polymers were characterized and the properties of paper sheet were detected. Results: Results indicated that the exponential phase of Komagataeibacter saccharivorans PE 5 and Acetobacter xylinum ATCC 10245 growth was detected during the first 48 to 144 hrs and during 168 hrs in productive media, respectively. The highest cellulose concentration and yield were obtained after 336 hrs by both tested strains being 11.11 gl-1 & 74.1% for Acetobacter xylinum ATCC 10245 and being 12.61 gl-1 & 84.11% for Komgataeibacter saccharivorans PE 5. The structure of BC produced from the tested strains was assayed by scanning electron microscope, fourier transform- infrared spectrum (FT-IR) and x-ray diffraction. It was revealed the diameter of thin ribbons ranged from 34.34 nm to 39.16 nm and the shape of FT-IR spectra for the two stains is similar to pure cellulose. The crystalinity index of A. xylinum ATCC 10245 is 54.14% and 52.76% for K. saccharivorans PE 5. Also, the properties of paper sheet from K. saccharivorans PE 5 cellulose increased about 1.19, 1.25, 1.33, 1.91 and 1.27 fold for wet tensile strength, dry tensile strength, degree of polymerization, brightness and opacity, respectively than A. xylinum ATCC 10245 cellulose.
Authors and Affiliations
Enas A. Hassan, Hemmat M. Abdelhady, Sohir S. Abd El-Salam, Sara M. Abdullah
Keywords
Related Articles
- EP ID EP354324
- DOI 10.9734/BMRJ/2015/18223
- Views 71
- Downloads 0
Antibacterial Activity of Croton bonplandianum (Bail.) Against Some Bacterial Isolates from Infected Wounds
Aims: The antibacterial activity of medicinal plants against human pathogens has been evaluated by a number of studies. However, a few research works have been done on pus cell pathogens. So this research work was aimed...
Clinical Characteristics, Antibiotic Resistance and Molecular Typing of Methicillin-Resistant Staphylococcus aureus in a Teaching Hospital at South Taiwan
Background: Methicillin-resistant Staphylococcus aureus (MRSA) has become an issue of public health worldwide. The clinical features, molecular epidemiology and antibiotic resistance of MRSA isolates from South Taiwan fr...
Analyzing Different Acetyl Co-A Metabolizing Enzymes as Potential Drug Targets against Mycobacterium tuberculosis
Mycobacterium tuberculosis, the causative agent of tuberculosis, is responsible for the deaths of million people around the globe. The scenario is worse than ever due to the emergence of drug resistant strains which are...
A Peculiar Phenomenon of Cold-shocked Bacteria Recovered on Sano- Gam Media
This study was conducted in order to display the peculiar appearance of bacteria which were recovered on Sano-Gam Media. The studied bacteria were Escherichia coli, Salmonella spp., Staphylococcus aureus, Proteus vulgari...
The Effect of IP-10 Level and HLA-DP/DQ Polymorphisms on Response to Nucleoside/ Nucleotide Analogues Treatment among Hepatitis B Egyptian Patients
Background: The efficacy of anti-viral drugs used in treatment of HBV is high but these drugs are expensive, associated with many side effects and drug resistance has evolved. Thus selection of patients with the highest...