The development and validation studies of RP-HPLC method – A review
Journal Title: World Journal of Pharmaceutical Sciences - Year 2016, Vol 4, Issue 1
Abstract
Today there are many different strategies stating High Performance Liquid Chromatographic Method Development as well as RP-HPLC Technique [8-9]. This review describes strategies for the systematic Development of High performance liquid chromatographic (HPLC) methods. HPLC is an analytical tool which is able to detect, separate and quantify the drug, and its various impurities and drug related degradants that can form on synthesis or storage. It involves the detection of the purity of a drug substance and facilitates in the development of its analytical method and also the various factors affecting them[13]. A number of chromatographic parameters have been evaluated in order to optimize the methods in the analysis of method development in HPLC and RP-HPLC. An appropriate mobile phase, column, column temperature, wavelength and gradient are developed, suitability, compatibility and stability of drug as well as degradants and impurities should as well be evaluated appropriately. In analytical method development of a drug, drug along with its impurities can be quantified and interpreted [22]. Force degradation studies are helpful in development and validation of stability-indicating studies, determination of degradation pathways of drug substances and drug products, discernment of the degradation products in formulations that are related to drug substances versus those that are related to non–drug substances[25] (e.g. related substances, excipients).
Authors and Affiliations
Manasa Padma M
Keywords
Related Articles
- EP ID EP194782
- DOI -
- Views 135
- Downloads 0
Management of Recurrent urinary tract infection in diabetic patient with Ayurveda and counselling- Case study
A female patient of 56 years came to the Diabetic screening and counselling OPD of Ayurveda and Unani Tibbia college & hospital in February 2015 with chief complaints of increased frequency of micturition and burning sen...
Reduction of cardiac effect of propranolol in lisinopril treated isolated rabbit heart
Background; Angiotensin converting enzyme inhibitors are widely used in hypertensive patients who may come across to a condition which require emergency treatments. Objective: Pharmacodynamic interaction of Propranolol a...
Herbal Hair Cosmetics - An Overview
The herbal cosmetics are known as the preparation containing variety of botanical sources which influence the functions of skin and provide nutrients necessary for the healthy skin or hair. Hair cosmetics are used to inc...
Galantamine as benzofuro-benzazepine alkaloid used in cognition and dementia
Galantamine is used to treat the symptoms of Alzheimer's disease (AD; a brain disease that slowly destroys the memory and the ability to think, learn, communicate and handle daily activities). Galantamine is in a class o...
A cadavericfoetal study of craniorachichisis totalis
Aim of the study is to study in detail about Neural tube defects and the importance of folic acid during antenatal period. Neurulation is the process whereby the neural plate forms the neural tube. Cranial end is called...