The development of methods for obtaining monoclonal antibody-producing cells
Journal Title: Advances in Hygiene and Experimental Medicine - Year 2016, Vol 70, Issue
Abstract
Monoclonal antibodies (mAbs) are biomolecules of great scientific and practical significance. In contrast to polyclonal antibodies from immune sera, they are homogeneous and monospecific, since they are produced by hybridoma cells representing a clone arising from a single cell. The successful technology was described for the first time in 1975; the inventors were later awarded the Nobel Prize. Currently, mAbs are broadly used as a research tool, in diagnostics and medicine in particular for the treatment of cancer or in transplantology. About 47 therapeutics based on monoclonal antibodies are now available in the US and Europe, and the number is still growing. Production of monoclonal antibodies is a multistage, time-consuming and costly process. Growing demand for these molecules creates space for research focused on improvements in hybridoma technology. Lower costs, human labor, and time are important goals of these attempts. In this article, a brief review of current methods and their advances is given.
Authors and Affiliations
Michał Skowicki, Tomasz Lipiński
Keywords
Related Articles
- EP ID EP220660
- DOI -
- Views 196
- Downloads 0
Nowe biomarkery w prognozowaniu przebiegu szpiczaka plazmocytowego
Szpiczak plazmocytowy jest złośliwą chorobą nowotworową charakteryzującą się niekontrolowaną proliferacją i akumulacją plazmocytów w szpiku kostnym, której zazwyczaj towarzyszy wydzielanie nieprawidłowego białka monoklon...
Possible involvement of 5-HT7 receptor in pathophysiology of affective disorders and action of antidepressant drugs
The 5-HT7 receptor has recently received considerable attention since its involvement has been implicated in cognitive disturbances, sleep and circadian rhythmicity disorders, anxiety and depression. At the cellular leve...
TGF-β1 i elastaza granulocytów w ocenie aktywności nieswoistych chorób zapalnych jelit. Badanie pilotażowe
Lysis of bacterial cells in the process of bacteriophage release – canonical and newly discovered mechanisms
The release of phage progeny from an infected bacterium is necessary for the spread of infection. Only helical phages are secreted from a cell without causing its destruction. The release of remaining phages is correlate...
Epigenetics and etiology of neurodegenerative diseases
Determination of specific gene profile expression is essential for morphological and functional differentiation of cells in the human organism. The human genome consists of 25–30 thousands genes but only some of them are...