The Diversity of Actinobacteria in Nihewan Basin
Journal Title: International Journal of Engineering and Science Invention - Year 2018, Vol 7, Issue 12
Abstract
Actinobacteria diversity was investigated in Nihewan basin by using two pair of Actinobacteria-specific primers for 16S rDNA clone library. The 8 different longitudinal archaeological sites we choose from Nihewan vary from 1,660, 1,640, 1,400, 1,320, 1,100, 900, 200 to 100 thousands year. We obtained 937 Actinobacteria positive clone, and 733 positive clone has the taxonomic status of Actinobacteria, distributed in 4 subclasses, 5 orders, 9 suborders, 25 families and 45 genera, other 204 clone are in the unclassified status, accounted for 21.8% of the total number of clone. Comprehensive analysis based on Species richness, Shannon index and Simpson index, demonstrating the species abundance among 8 sample, and the highest Actinobacteria diversity were in sites of 1,400 thousands year while lowest in 1,660 thousands year. Furthermore, Nocardioides, Corynebacterium and Dietzia decreased from 1660 thousands year ago to 100 thousands year ago. While Aciditerrimonas, Conexibacter increased from 1660 thousands year ago to 1320 thousands year ago, however, the abundance decreased from 1320 thousands year ago to 100 thousands year ago, excepting the site of 200 thousands year ago. It is been studied that, the structure of microorganism may be linked with the environmental change.
Authors and Affiliations
Liuying Tian, Hui Tang, Liping Zhang, Brajesh K. Singh, Kellys. Ramirez, Vigdis Torsvik, David A. Wardle
Keywords
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