THE EFFECT OF BACILLUS Subtilis ON THE BACTERIAL CONTENT IN RABBITS CAECI
Journal Title: Journal of Applied Veterinary Sciences - Year 2020, Vol 5, Issue 4
Abstract
In the present study, the effect of B. subtilis (107cfu/gm) supplemented to the basal diet of two groups of New Zealand rabbit of 28 days (n=15/group) on the count of commensal microflora ( Bifidobacteria and Lactobacilli spp.) and some opportunistic pathogens (E. coli and C. perfringens type A) in the rabbit caeci was investigated. Results indicated that the count of Bifidobacteria and Lactobacilli spp. are significantly increased at all intervals (7, 14, 28 and 40 days post supplementation) in the supplemented group compared with the non-supplemented group with P< 0.001. On the other hand, there is a significant decrease in the count of E. coli and C. perfringens especially at 40 days post supplementation from (5.8 log 10 CFU ±0.06) to (3.1 log 10 CFU ±0.07) and from (4.8 log 10 CFU ±0.09) to (2.3 log 10 CFU ±0.09) respectively with P< 0.001. B. subtilis proved to be tolerant to the simulated gastric and intestinal juice for one hour (100% tolerance). However, after two hours, the tolerance to the gastric juice decreased to 70%, with no effect of the artificial intestinal juice on the viability of the organism. In vitro, the antimicrobial effect of B. subtilis on C. perfringens type A by well diffusion method showed an inhibition zone of 10 mm. Results of in vitro effect of B. subtilis on C. perfringens count showed that at 103 dilutions of C. perfringens, its count was reduced 2.1×105 to 3×104 after adding B. subtilis, while at dilution of 102, the count was reduced from 2.4×105 to 7×103cfu/ml after adding B. subtilis. It could be concluded that B. subtilis can be used as a probiotic in rabbits ration due to its ability to increase the commensal microflora count beside its antibacterial effect against some opportunistic pathogens
Authors and Affiliations
Eman, F. Farag and G. A. Abdel-Alim
Keywords
Related Articles
- EP ID EP689171
- DOI 10.21608/JAVS.2020.117997
- Views 185
- Downloads 0
Genetic and Pathological Characterizations of Gumboro (IBD) in Chickens
Gumboro, or infectious bursal disease (IBD), represents a significant threat to poultry worldwide due to its genetic and pathogenic variations. Viruses can mutate and produce new variants, which can have an impact on the...
Bacteriological and Molecular Comparative Study between Staphylococcus aureus Isolated from Animals and Human
Staphylococcus aureus represents a serious health hazard on both animals and humans. The main goal of the present work was to compare between S. aureus isolated from animal and human origin. This was achieved through stu...
Effect of Applying Clove and Cinnamon Essential Oils to Milk Rice Pudding in Controlling Bacillus cereus and Bacillus subtilis Growth with Respect to the Sensory Traits
Milk rice pudding (MRP) is a commercial and popular dairy dessert, but owing to its characteristics and valuable ingredients, it may be contaminated by many pathogenic and spoilage microorganisms. So, this study aimed to...
Effectiveness Of The Pigeon Pox Vaccine On The Chicken Vaccinated With Avian Influenza Vaccine
Evaluation of the effectiveness of pigeon pox (PP) vaccine in improving the required immunity to avian influenza (AI) virus vaccine in birds and reducing its shedding after the challenge was the object of this study. Spe...
Studies on some Welfare Aspects of Broilers Reared Under Different Stocking Densities
Nowadays, there is much concern expressed about stocking density as it is related to the well-being and welfare of broilers, and to define some welfare aspects of broilers in an environmentally uncontrolled conventional...