The Effect of Curcuma domestica Val Extract on Phagocytic Activity of Macrophage in Listeria monocytogenes Inoculated BALB/C Mice
Journal Title: SAINS MEDIKA (Journal of Medicine and Health) - Year 2012, Vol 4, Issue 1
Abstract
processing antigen in such a way that emerge interactions among immune cells. Listeria monocytogenes infection often used as an example to demonstrate the immune response to the intracellular bacteria. Curcuma has been proven in vitro to have immunomodulatory effect to improve the function of T cell, NKcell, macrophage, and splenocyte. Thus, it is presumed that curcuma extract may influence macrophage phagocytic activity. The aim of this research is to find out whether there is a difference between macrophage of mice inoculated with Listeria monocytogenes after treatment of curcuma extract in stepped dose compared with control. Design and Method: This laboratory experimental research used a Post-Test Only Control Group Design method. The samples were 20 males of balb/c mice. The mice were divided into 4 groups adopted for a week before the treatments for 20 days. The groups consist of control group, group I (receiving 0.13mg/day of curcuma extract), group II (receiving 0.26 mg/day of curcuma extract, group III (receiving 0.52mg/day curcuma extract). Mice were inoculated with Listeria monocytogenes on day 15. After isolation, the macrophage was desensitized using latex beads and observed under the microscope. The data were analyzed using One way Anova test was followed by Post hoc test using SPSS 15 programme. Result: Index of macrophage phagosytosis in control group, group I, II,II were 0.914±0,27, 1.940±0,42, 2.914±0.56, 1.30±0.31 respectively. One Way Anova test showed a difference among each of groups P<0.05 except for group I and II. Conclusion: Curcuma treatment can increase macrophage phagocytic activity on balb/c mice inoculated with Listeria monocytogenes (Sains Medika, 4(1):63-70).
Authors and Affiliations
Dini Pangestika, Erna Mirani, Imam D. Mashoedi
Keywords
Related Articles
- EP ID EP234339
- DOI -
- Views 65
- Downloads 0
The Effect of Honey on Plasma Malondialdehyde (MDA) Level onAlloxan-Induced hyperglycemic Rats An Experimental studies in rats Galur Wistar White Males
Malondialdehyde (MDA) is the end product of lipid peroxidation and a marker of free radicals. Honey is a safe sweetener proven to lower blood glucose level and contains flavonoids, vitamin A, C, E as a source of antioxid...
The Correlation of Exercise and Range of Motion (ROM) on Elderly Knee Observasional Study on Elderly People in Pedurungan Semarang
Background: In aging process, decreasing of musculosceletal capabilities will reduce physical activity and excercise, as well as Range Of Motion (ROM). In order to maintain the normality of ROM, joints and muscles should...
Correlation between Attitude of Midwive and Support Cadre in the Behavior of Vitamin a Postpartum Mother Giving Primary Health Center Klaten Region
The Program to prevent vitamin A deficiency in Indonesia includes the administration of vitamin A for post partum mothers by giving a high dose of vitamin A (@ 200,000 international standard). However, midwives sometimes...
The Effect of Tempeh on the Ability of Macrophage Phagocytosis An experimental study in male Balb/c mice
Background: Consuming tempeh regularly increases the levels of isoflavones in the our body influencing the IFNγ and macrophage activating factor (MAF) that lead to the in increase in the ability of macrophage phagocytosi...
Effect of White Turmeric Extract (Curcuma zedoaria) Using Zam-zam Solvent Compare with Ethanol Solvent Against Breast Cancer Cell T47D
Introduction: Curcuma zedoaria is one of the herbal plants potentially protects and cures many diseases, particularly as anti-cancer and anti-tumor. Anti-cancer active compounds of it are flavonoid, phenolic, and saponin...