The effect of different agitation modes on bacterial cellulose synthesis by Gluconacetobacter xylinus strains
Journal Title: Acta Scientiarum Polonorum Zootechnica - Year 2015, Vol 14, Issue 1
Abstract
The present study focus on the research concerning the effect of various agitation modes on bacterial cellulose (BC) synthesis by different strains of Gluconacetobacter xylinus. The BC biosynthesis process was performed for 7 days at 28°C in 50 mL plastics tubes using a roller shaker or in 50 mL plastics tubes equipped with disks made of metal ferrite or polycarbonate using a shaker roller, or in 100 mL glass flasks on an orbital shaker. All the cultures were carried out in the agitated conditions with a variety of mixing speeds. After 7 days of incubation, the weight of the synthesized BC, density and pH of the culture medium were determined. It was found, that the stirring speed during the cultivation was the one of the most important parameters affecting on the biosynthesis process of bacterial cellulose. It was shown, that, depending on the stirring speed, BC formed the different size spherical forms or was produced as a mass with irregular shapes. Higher density of G. xylinus cells was found in culture agitated with higher stirring speed. It was also determined, that the highest weight of BC was obtained in culture agitated at the speed of 150 rpm on the orbital shaker.
Authors and Affiliations
Anna Żywicka, Dorota Peitler, Rafał Rakoczy, Maciej Konopacki, Marian Kordas, Karol Fiałkowski
Keywords
Related Articles
- EP ID EP74931
- DOI -
- Views 99
- Downloads 0
INFLUENCE OF THE WEIGHT OF HATCHING EGGS ON THE HATCHABILITY INDICES AND ON THE BODY WEIGHT OF GEESE IN REARING AND AFTER FATTENING WITH OATS
Goose husbandry is characterized by considerable diversity of goslings in terms of body weight. These discrepancies result from a long laying performance. During different reproductive seasons females lay eggs of differe...
ANALYSIS OF THE MILK YIELD AND MILKING FREQUENCY OF POLISH BLACK-AND-WHITE HOLSTEIN-FRIESIAN COWS HOUSED IN A BARN WITH AN AUTOMATIC MILKING SYSTEM (AMS)
The aim of the study was to analyse milk yield and the frequency of use of a milking robot by Polish Holstein-Friesian cows. The study was carried out on a farm where a double-stall Astronaut milking robot from Lely was...
HISTOPATHOLOGICAL CHANGES OF THE PECTORAL MUSCLE IN PHEASANTS (PHASINUS COLCHICUS; PHASINUS C. MONGOLICUS)
The aim of investigation was the frequency of histopathological changes of the m. pectoralis superficialis of game and Mongolian pheasants of different ages. The samples for analyze from pectoral muscle were taken after...
Application aspects of animal and human genomics
Currently, extensive investigations are being carried out in the area of mitochondrial genomics. Mitochondrial DNA is used in many fields of science, e.g. evolutionism, phylogeography, anthropology, archaeology, forensic...
Effect of some factors on sex of the calf born, and of sex of the calf on performance of dairy cows
Records of 384965 parturitions and milk and reproductive performance in subsequent production cycles of 114 526 Black-and-White Polish Holstein-Friesian cows were collected from the SYMLEK database. The animals represent...