The effect of different agitation modes on bacterial cellulose synthesis by Gluconacetobacter xylinus strains
Journal Title: Acta Scientiarum Polonorum Zootechnica - Year 2015, Vol 14, Issue 1
Abstract
The present study focus on the research concerning the effect of various agitation modes on bacterial cellulose (BC) synthesis by different strains of Gluconacetobacter xylinus. The BC biosynthesis process was performed for 7 days at 28°C in 50 mL plastics tubes using a roller shaker or in 50 mL plastics tubes equipped with disks made of metal ferrite or polycarbonate using a shaker roller, or in 100 mL glass flasks on an orbital shaker. All the cultures were carried out in the agitated conditions with a variety of mixing speeds. After 7 days of incubation, the weight of the synthesized BC, density and pH of the culture medium were determined. It was found, that the stirring speed during the cultivation was the one of the most important parameters affecting on the biosynthesis process of bacterial cellulose. It was shown, that, depending on the stirring speed, BC formed the different size spherical forms or was produced as a mass with irregular shapes. Higher density of G. xylinus cells was found in culture agitated with higher stirring speed. It was also determined, that the highest weight of BC was obtained in culture agitated at the speed of 150 rpm on the orbital shaker.
Authors and Affiliations
Anna Żywicka, Dorota Peitler, Rafał Rakoczy, Maciej Konopacki, Marian Kordas, Karol Fiałkowski
Keywords
Related Articles
- EP ID EP74931
- DOI -
- Views 111
- Downloads 0
Effect of caponizing Yellowleg partridge (Ż-33) cockerels on body weight and meat quality
The aim of the study was to determine the effect of caponization on the body weight and selected meat quality parameters of Yellowleg Partridge (Ż-33) cockerels. Ż–33 cockerels were randomly assigned to two groups with 4...
The sexual activity and ejaculate traits of Duroc, Hampshire and Pietrain boars and their crosses
The experiments were conducted on 31 boars, including: 5 Duroc, 5 Hampshire and 7 Pietrain boars, as well as 9 Duroc x Pietrain and 5 Hampshire x Pietrain hybrids. Two series of measurements were performed at three-month...
Conformation analysis of horses used in equine-assisted activities at Polish hippotherapeutic centers
The objective of the research was to determine the basic conformation traits of horses of different breeds performing hippotherapeutic service at chosen equine therapy centers. The research material included a total of 4...
Effect of changing the cow's milking system on daily yield and cytological quality of milk
The aim of the study was to analyse the effect of changing the milking system (from a milking parlour to automatic milking) on daily yield of cows and cytological quality of their milk. GLM and FREQ procedures of the SAS...
TIME OF LYING IN THE HORSE WITH REGARD TO ITS SEX AND SYSTEM OF MANAGEMENT
The objective of the study was to determine demands for lying in adult horse males and females, as well as in females maintained under various systems. 43 horses were studied in three centres. Time of lying was measured...