The effect of different physical forms of rapeseed as a fat supplement on the activity of some enzymes in the duodenal chyme of dairy cows
Journal Title: Journal of Animal and Feed Sciences - Year 2014, Vol 23, Issue 4
Abstract
Studies on nutritional regulation of digestive enzymes in ruminants are scarce. Fat supplementation of diets for dairy cows changes the supply of nutrients for absorption and transport. The aim of this experiment was to study the effect of the physical form of rapeseed (Brassica napus) fat on the duodenal enzyme profile in dairy cows. Four mid-lactation Danish Holstein dairy cows were submitted to an experiment with a 4 × 4 Latin square design and fed four rations containing 50% forage and 50% concentrate on a dry matter basis. The four rations were: a control ration with rapeseed meal (low fat) and three fat-supplemented rations with either rapeseed cake (RSC), whole cracked rape seed (WCR), or rapeseed oil (RSO). The correlation coefficients among duodenal enzyme activities and the relationship between α-amylase and protease activities were examined. Diurnal samples were taken from the duodenum, frozen immediately and stored frozen (–20°C) before analyses. Additional fat increased the soluble protein content in duodenal chyme (P < 0.05). Neither α-amylase nor the protease or soluble sugar concentrations were affected by the treatments. Lipase activity increased for RSO compared with the control. The relationship between α-amylase and protease activities showed a parabolic behaviour (r = 0.77; P = 0.061). In conclusion, inclusion of rapeseed oil in the ration of dairy cows under the experimental conditions increased the soluble protein concentration and lipase activity in duodenal chyme.
Authors and Affiliations
A. Moharrery, M. Brask, M. R. Weisbjerg
Keywords
Related Articles
- EP ID EP75828
- DOI -
- Views 96
- Downloads 0
Effects of dietary fish oil on NFκ B gene expression and related signaling in spleen of chickens stimulated with lipopolysaccharide
The study was conducted to investigate the effects of fish oil and maize oil on nuclear factor kappa B (NFκB) gene expression and the downstream pathways of intracellular signaling in spleen of chickens after lipopolysac...
Evaluation of microalgae as sources of digestible nutrients for monogastric animals
The study was carried out to evaluate three microalgae as potential nutrient sources in diets for monogastric animals. In a digestibility experiment with adult mink (Mustela vison), the microalgae Nannochloropsis oceanic...
A new set of endogenous reference genes for gene expression studies of porcine stomach
Gene expression analysis by Real-Time PCR requires careful selection of endogenous reference genes to obtain accurate results. We evaluated usefulness of six reference genes to expression studies of porcine stomach. We s...
Genetic parameters for lactose percentage in the milk of Polish Holstein-Friesians
Genetic parameters for lactose percentage in the milk of Polish Holstein-Friesian cows were estimated using 48,859 test day records from six lactations of 8,418 cows and the Gibbs sampling method. The multiple-trait rand...
Responses of mammary amino acid metabolism and aminopeptidase N gene expression to duodenal soyabean small peptides and infusion of free amino acids in lactating goats
The duodenal perfusion technique was used to determine whether small peptides were more effective than free amino acids (FAA) for milk protein synthesis and to investigate the effects of infusing soyabean small peptides...