The Effect of Endurance Exercise and Adenosine Consumption on UCP-1 Gene Expression in the Visceral Adipose Tissue of Obese Male Rats
Journal Title: Iranian Journal of Diabetes and Obesity - Year 2018, Vol 10, Issue 2
Abstract
Objective: Reducing white adipose tissue is an important strategy for treatment of obesity and its related metabolic disorders. The purpose of this study was to determine the effect of endurance exercise and adenosine consumption on the expression of selected genes of thermogenic pathway in the visceral adipose tissue of obese male rats. Materials and Methods: Twenty male wistar rats were fed on a high-fat diet (40 % fat) 12 weeks to get mean weight 319±30 gr. The rats were selected randomly in four groups; control-saline, control-adenosine, exercise-adenosine and exercise-saline. The adenosine groups received 0.2mgr/kg adenosine 12 weeks (seven days a week), 3 hours before exercise. The exercise schedule included running on the turning wheel without slope, performed 12 weeks, five sessions a week, with the speed of 20-25 meters per minute for 15-31 minutes. About 48 hours after the last exercise session, the rat’s kidneys were removed and frozen immediately. In order to measure the relative expression of UCP-1 gene, the method of Real Time (RT) - PCR based on SYBR-Green dye was used. The gathered data was studied using the statistical method of two-way analysis of variance. Results: Twelve weeks of endurance exercise with medium intensity and adenosine consumption resulted a significant increase of UCP-1 gene expression (P-value: 0.001 and P-value: 0.005 accordingly). Nevertheless, the collective effect of exercise and adenosine is indicative of insignificant difference in changes of the relative expression of UCP-1 gene in visceral adipose tissue. Conclusion: Endurance exercise and adenosine consumption independently led to an increase in UCP-1 gene expression in the visceral adipose tissue of obese male rats Nevertheless, the collective effect of exercise and adenosine is indicative of insignificant difference in changes of the relative expression of UCP-1 gene in visceral adipose tissue. It is probable that some mechanisms be activated through the collective effect of exercise and adenosine that reduce the synergic effect of exercise and adenosine.
Authors and Affiliations
Amene Barjaste Yazdi, Hassan Matin Homaee, Maghsood Peeri
Keywords
Related Articles
- EP ID EP636058
- DOI -
- Views 62
- Downloads 0
Fasting Blood Sugar, Glucose Challenge Test and One- Two Hour Glucose Tolerance Test in Diagnosis of Gestational Diabetes in Women without Risk Factor
Objective: Gestational diabetes mellitus (GDM) is the most common metabolic disorder during pregnancy. This study aimed to compare the prevalence of GDM positive screening tests with Fasting Blood Sugar (FBS) and Glucose...
Whey Protein Supplementation is Associated with Antioxidant Markers Following Severe Eccentric Contractions in Obesity
Objective: Intense muscle contractions are associated with oxidative stress and immune system deficiency, especially in the presence of obesity. This study aimed to determine the effect of whey protein supplementation fo...
Impact of Magnesium Deficiency on Pancreatic β-Cell Function in Type 2 Diabetic Nigerians
Objective: Pancreatic -cell dysfunction is described to be present at the diagnosis of type 2 diabetes mellitus (T2DM) and progressively deteriorated with disease duration. However, its progression is variable and poten...
Distribution of ABO and Rh Blood Groups among Diabetes Type 2 Patients in Yazd Diabetes Research Center (2015-2016)
Objective: Blood group as a genetic trail can be associated with type 2 diabetes. The aim of this research was to determine the distribution of ABO frequency and RH blood groups in type 2 diabetic patients referred to Ya...
Feto-maternal Outcome in Patients with Gestational Diabetes Mellitus in Western India: A Two Years Follow up Study
Objective: Untreated gestational diabetes mellitus (GDM) may lead to increased risk of macrosomia, congenital anomalies, unexplained stillbirth, hypoglycemia and jaundice in newborns. This prospective study was conducted...