The effect of exhaustive exercise on the concentration of purine nucleotides and their metabolites in erythrocytes
Journal Title: Biology of Sport - Year 2008, Vol 25, Issue 1
Abstract
In this study we tried to obtain a complete overview of purine nucleotide metabolism in erythrocytes before and during an incremental, intermittent exhaustive exercise bout protocol for sportsmen (high-performance rowers) and untrained, healthy, active volunteers. Erythrocyte levels of the main nucleotides (ATP, ADP, AMP, GTP, GDP, GMP, IMP, NAD+ and NADP+), nucleosides (Ado, Guo, Ino) and the base Hyp were measured using the HPLC method. The parameters that can be deducted from their concentrations: TAN, TGN and AEC, GEC expressed by the ratio of high/low energy nucleoside phosphates were calculated. The effects of graded rowing exercise on the concentration and metabolism of erythrocyte purine and pyridine nucleotides and the activity Na+, K+-ATPase in both trained and untrained individuals. In the group of sportsmen, ATP, ADP and AMP concentrations were decreasing during the effort (for ATP rs=-0.65). However, ADP and AMP concentrations were significantly lower only during recovery (p<0.0001). We observed a negative correlation (rs=-0.72) between the ATP concentration and the Ado concentration during maximal effort. In the sportsmen group, NAD+ concentration was decreasing during the effort (rs=-0.68). Lower activity of Na+, K+-ATPase in erythrocytes was significantly correlated with the decrease in ATP concentration after maximal effort in sportsmen. This study confirms that erythrocyte energy metabolism depends on the body’s adaptation to effort and the physical efficiency of the participants.
Authors and Affiliations
E Skotnicka, I Baranowska-Bosiacka, W Dudzińska, M Suska, R Nowak, K Krupecki, AJ Hłyńczak
Keywords
Related Articles
- EP ID EP58928
- DOI -
- Views 110
- Downloads 0
MEASUREMENT OF REACTION TIME IN QWAN KI DO
The aim of this study is to identify the simple and choice reaction time to visual stimuli, for the upper limbs, in beginner and advanced practitioners of the Qwan Ki Do martial art, and also to identify differences betw...
Variation of explosive force at different times of day
AIM: The purpose of this study was to compare the explosive force and electromyographic (EMG) activity at three different times of the day. METHODS: Thirty healthy subjects took part in the study, and carried out two max...
Effects of training status and different treadmill exercises on the activity of complement receptor type 1 of erythrocytes
The aims of this study were to investigate the effects of training status, different intensities, durations and modes of exercises on the activity of complement receptor type 1 (CR1) of erythrocytes. Fifteen sedentary ma...
A PILOT STUDY ON HOW DO ELITE SURFSKI PADLLERS MANAGE THEIR EFFORT AND HYDRATION PATTERN IN THE HEAT
To investigate thermal response, hydration behaviour and performance over flatwater kayaking races in tropical conditions (36.8°C and 68 % rh). Five internationally-ranked subjects participated in the 2012 Surfski Ocean...
Kinetic energy transfer during the tennis serve
Several studies have established the pattern used in the over arm hitting and throwing movements, however to date there has not been one which statistically expresses the Kinetic Link Principle of the tennis serve. The m...