The Effect of Glycine Treatment on Histomorphometric Changes in the Diabetic Rat Retina
Journal Title: International Journal of Medical Research & Health Sciences (IJMRHS) - Year 2016, Vol 5, Issue 6
Abstract
iabetic retinopathy is a major cause of blindness and one of its possible mechanisms is impairment in proteins and amino acids synthesis due to impairment in glucose synthesis. So far, different amino acids have been used to improve diabetic retinopathy. However, no research has been done till now about the effect of glycine on histological changes of diabetic rats retina. So, this is the main goal of the current study. 40 adult male Wistar rats weighing approximately 200- 250 g were prepared and divided into 4 groups of 10 each, which include: the first group (the rats were kept without receiving any material), the second group (healthy, the rats were received glycine at concentration of 130 mM, 1 %w/v), the third group (diabetic, the rats were kept without receiving any material), the fourth group (diabetic, the rats were received glycine at concentration of 130 mM, 1 %w/v). All rats were anesthetized after 16 weeks The separated retina was immersed in glutaraldehyde 4% for 3-4 h. Retina tissue was rised with buffer, post fixed in osmium tetroxide 1% and dehydrated through a graded ethanol series. The tissue was then placed in a mixture of propylene oxide and TAAB resin and transferred in pure resin. Semithin sections was prepared and viewed under light microscope (LM). Ultrathin sections were prepared, stained, and Viewed under TEM. Studies and evaluation of the thickness of IPL, INL, OPL, ONL, PSL and GCL. After preparing slides from the samples and staining with toluidine blue stain, they were examined and compared with light microscopy. The results showed that a significant decrease in the thickness of PSL and ONL and a significant increase in the thickness of IPL were observed in the group received glycine compared to the normal group (P≤0.05). There was a decrease in the thickness of all layers except PSL and IPL in the diabetic group compared to the normal group. These changes were not statistically significant. The thickness of whole retina and its layers were increased significantly in diabetic group received glycine compared to other groups (P≤0.05). Glycine increases the thickness of retinal layers in the retinopathic group through its anti-diabetic properties as well as its influence on improvement of protein synthesis in retina; so, treatment with glycine is recommended to improve different layers of retina in diabetic patients with retinopathy.
Authors and Affiliations
Soghra Gholami| Department of Basic sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran Corresponding Email: gholami@shirazu.ac.ir, Mohammad Reza Rostamzad| Department of Basic sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Keywords
Related Articles
- EP ID EP11753
- DOI -
- Views 252
- Downloads 10
150-Cavity Synchronization by Extended Loop in Neuraminidase of 2009 H1N1 Influenza Type A Virus: A Simulation Study
Neuraminidase (NA) is a novel drug target for antiviral inhibitors. In this druggable target, a cavity adjacent to the active site exists and is known as the 150-cavity. NA is divided into two groups: Group 1 and Group...
Development a Model of the Relationship of Psychological Well-Being with Self-Efficacy, Self-Esteem and Psychological Hardiness in Cancer Patients of Benign Brain Tumors
Brain tumors are common causes of mortality and disabilities in human societies. With the all-round advances in the fields of diagnosis and treatment as a group and team and interdisciplinary is an undeniable necessity....
Diagnostic accuracy of Digital Bite Wing radiography in interproximal carious lesion detection of posterior teeth
Background and purpose: Dental decay is the most prevalent infectious disease. One of the dentists’ major disturbances is accurate diagnosis of small proximal decay in posterior teeth. Different diagnostic methods hav...
The comparison of mental health and happiness and a life expectancy in children of veterans and non-veterans in Shahed schools of Qom, 2015
According to the psychological and social changes of adolescence, people in relationships with family, school and peers experience conflict. In shaping attitudes and views of adolescent, emotional aspects of relationshi...
Effectiveness of problem-solving skills training in the attributional style and socialization of divorced women
This research was a pretest-posttest quasi-experimental study design with a nonequivalent control group, which was conducted with the aim of investigating the effectiveness of problem-solving skills training in the attr...