The Effect of Granulocyte Colony Stimulating Factors (GCSF) on Expansion of CD34+ Hematopoietic Stem Cells
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 12, Issue 5
Abstract
Stem cells are alternatives therapeutic treatment, mainly for diseases that cannot be treated by medical or surgical treatment. CD34+ hematopoietic stem cells which is isolated from autologous peripheral blood cell is a good source because it’s lowering immune rejection However, the limit volume of peripheral blood can be taken from a patient, limit the total number of CD34+ cell and therefore will be inadequate for therapeutic efficacy. In the previous addition of GCSF, TPO and SCF to CD34+ culture increased the number of CD34+ cell. But the addition of several cytokines will increase the cost of culture become a burden for patients who are seeking for stem cell therapy. In this study we examined the effect of GCSF on the expansion of CD34+ cell. For that purpose, we isolated and expanded CD34+ cell from peripheral blood using GCSF. As a control cultures, we used GCSF, TPO and SCF addition. Both culture was incubated for 10 days, and then counted the number of CD34+ cells. The results showed the amount of CD34+ cells can be increased only with GCSF. When compared to the control group, the number of CD34+ cells treated with GCSF were lower than which are an average 51,250 cells compare to 3,300,000 cells, respectively. There is a significant difference between the number of cd34 cells treated only with GCSF and the control group (p=0.04). Furthermore, the differentiation potential of CD34+ /GCSF expanded cells should be performed. In conclusion, GCSF alone has positive effect in increasing CD34+ cells number although the increase is lower compared to if all the treatment is combined.Human tissue has varying levels in regeneration capacity, even some of musculoskeletal tissues such as the cartilage and ligament have no ability to regenerate at all. In some condition when that tissues had trauma or diseased, they cannot regenerate. Until now, medical therapy has not been able to make growth or produce new tissue that tissue disorders are often end with surgical therapy such as replacement surgery. The solution is to make new tissue by regeneration therapy. Tissue engineering technique using stem cell could be the promising alternative therapy because of its ability to regenerate new tissue. Stem cells are cells that have ability to renew and multiply themselves and form cells and tissues structuring the organism body. Stem cells have some superior characteristics, namely plasticity produce regenerative tissue and prevent degeneration. Stem cells can be categorized into embryonic stem cells and tissue stem cells. There are some limitations in using them such as ethical problem and teratoma risk. Tissues stem cells found in some tissues or organs. There are 2 types of tissue stem cells, mesenchymal stem cells and hematopoietic stem cells. Mesenchyme stem cells are potential sources of tissue engineering. However, the application of mesenchyme stem cells faces some limitations in sampling process and its nature. The sampling process causes pain and complication such as infection and sepsis. The other limitations are the characteristic of mesenchyme stem cells such as the number of cells, range of age, proliferation and its differentiation related to age of the donor. Hematopoietic stem cells are favorable because its characteristic, namely easy taking, low complication risk, and have differentiated potential without being affected by the age of the donor. Hematopoietic stem cells are progenitor cells forming blood cells-lymphoid cells and myeloid cells. Cells, that come from bone marrow and blood respectively. Hematopoietic stem cells have pluripotent and plasticity so that they can form nonhematopoietic cells. The change of lineage is assumed to undergo some mechanism namely trans differentiation, dedifferentiation, and cell fusion. Hematopoietic stem cells can move toward the target tissues and form new cells and tissue. Major source of HSC is CD34+ cells, the other is CD133. Today, Hematopoietic Stem Cells (HSC) are clinically efficiently used for blood cancer therapy. More research indicates other uses of HSC for non-hematopoietic tissue such as for musculoskeletal tissue especially for ischemic chronic therapy in legs and osteogenesis imperfecta.
Authors and Affiliations
Basuki Supartono, Bintarti Yusriana, Muttia Amalia
Keywords
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- EP ID EP585912
- DOI 10.26717/BJSTR.2019.12.002304
- Views 122
- Downloads 0
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