The Effect of Hydroalcoholic Extract of Nigella sativa on Plasmodium berghei-Infected Mice: An Evaluation of Immune Deviation and Serum Levels of Interferon Gamma (IFN-γ) and Interleukin 4 (IL-4)
Journal Title: Journal of Clinical and Diagnostic Research - Year 2019, Vol 13, Issue 3
Abstract
ABSTRACT Introduction: In tropical countries, malaria is known as one of the most widespread infectious diseases with an estimated 212 million cases globally. Treatment of malaria has become more difficult when the drug resistance appears against parasites. Therefore, safe and effective new drugs are highly required. Because of simplicity, lower cost, low rate of serious complications, and greater tolerability, traditional medicine is an important source for new drugs. Aim: To evaluate the anti-malaria and immune modulatory effects of Nigella sativa against Plasmodium berghei in vivo. Materials and Methods: This study was conducted in the Department of Parasitology, the Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran during January 2014 to January 2015. N. sativa powder was macerated in methanol and filtered with Bokhner hopper, and solvent was separated in a rotary evaporator. In the result of toxicity test, the 450 mg/kg was determined as maximum dose with minimum toxicity. Antimalarial efficacy and interferon gamma (IFN-γ) and Interleukin 4 (IL-4) cytokines level were investigated in five groups of P. berghei-infected BALB/c mice. The percentage of parasitaemia and surveillance were also evaluated. Results: The results of our study showed no toxicity, even at high concentration of herbal extract. A significant reduction in the percentage of parasitaemia was not observed in the treatment group. N. sativa-treated infected mice showed a significant increase in the IFN-γ but not IL-4 serum level, In addition, no higher surveillance was observed in the mice group treated with N. sativa compared to treated mice. Conclusion: Hydroalcoholic extract of N. sativa has the weak efficiency against P. berghei. However, there is requirement to evaluate the immune mechanism and also find the major component of this herbal extract by further studies.
Authors and Affiliations
Khosrow Hazrati Tappeh, Mahmoud Ghaderi, Shahram Seyedi, Peyman Mikaili, Arash Aminpour
Keywords
Related Articles
- EP ID EP582595
- DOI 10.7860/JCDR/2019/38186.12681
- Views 100
- Downloads 0
Effect of Ethyl Acetate Extract of Melothria Perpusilla on Oral Glucose Tolerance Test in Albino Rats
Introduction: Diabetes mellitus is a group of common metabolic disorders sharing the phenotype of hyperglycaemia. Certain disadvantages like side effects or less efficacy limit the optimal use of antidiabetic drugs. Aim:...
The Effect of a 16-week Home-based Aerobic Exercise Program on Serum High-sensitivity C-Reactive Protein (Hs-CRP) and Insulin Resistance in Polycystic Ovary Syndrome
ABSTRACT Introduction: Polycystic Ovarian Syndrome (PCOS) is one of the most common hormonal disorders that affect 10 to 15% of women of reproductive age. Women with PCOS are at risk for complications such as pregnancy p...
Vitamin D in Treatment-Resistant Carpal Tunnel Syndrome Patients with Hypovitaminosis D
Introduction: Vitamin D significantly effects health. Extraskeletal effects of vitamin D play an important role. Aim: To study effect of treatment with vitamin D in intractable Carpal Tunnel Syndrome (CTS) patients with...
Effect of Obesity on Pulmonary Functions among the Adolescent Students of a Private University in Malaysia
ABSTRACT Introduction: Obesity is a risk factor for many conditions including respiratory disorders. However, studies investigating pulmonary functions in obese adolescents from Malaysia are few in number. Aim: To invest...
Correlation between Chronic Obstructive Pulmonary Disease and Cardiovascular Abnormality: A Cross-sectional Study
ABSTRACT Introduction: Cardiovascular manifestation is one of the common co-morbidity and also the complication of Chronic Obstructive Pulmonary Disease (COPD). Electrocardiography and echocardiography are rapid, non-inv...