The Effect of Hypoxic Mesenchymal Stem Cell Secretome on TNF-Α and MCP-1 Gene Expression
Journal Title: International Journal of Multidisciplinary Research and Analysis - Year 2023, Vol 6, Issue 09
Abstract
Diabetes mellitus (DM) type 2 is a metabolic disorder with the occurrence of insulin receptor resistance and reduced ability of pancreatic β-cells to secrete insulin, there will be an increase in adipose tissue which causes hypertrophy and hyperplasia. This situation triggers inflammation involving the role of pro-inflammatory cytokines and chemokines such as TNF-α (Tumor Necrosis Factor-Alpha) and MCP-1 (Monocyte Chemotactic Protein-1). Cell-based therapies such as the administration of hypoxic mesenchymal stem cell secretom offer a new paradigm in the management of T2DM. This study aims to determine the effect of hypoxic mesenchymal stem cell (SSPM) administration on TNF-α and MCP-1 gene expression in STZ-induced obese DM rats. This research is experimental with a post-test-only control group design approach. The subjects of the study were 20 male Wistar rats which were randomly divided into 4 groups. Group K1 was given standard feed and distilled water. Group K2 subjects with obesity mellitus type 2 with 0.9% NaCl. Groups K3 and K4, subjects with type 2 obesity mellitus, were treated with secretom doses of 250 μl and 500 μl. The study was conducted at the Sultan Agung Stem Cell Research Institute Laboratory for 21 days by taking blood for examination of TNF-α and MCP-1 gene expression using the PCR method. The result showed that the lowest average TNF expression was in the K4 group (3.19 pg/ml) and the lowest mean MCP-1 expression was in the K4 group (1.57 %). One-way ANOVA test showed a significant difference in TNF- expression with a value of p=0.006 and MCP1 expression showed a significant difference p=0.000 (p<0.05). The Administration of hypoxic mesenchymal stem cell (SSPM) secretom significantly reduced TNF-α and MCP-1 gene expression in STZ-induced obese DM rats.
Authors and Affiliations
Ihdina Hanifa Hasanal Ibrahim, Agung Putra, Titiek Sumarawati
Keywords
Related Articles
- EP ID EP721263
- DOI 10.47191/ijmra/v6-i9-75
- Views 19
- Downloads 0
Effectiveness of QRIS on Motor Vehicle Tax Payment Compliance through the Technology Acceptance Model (TAM) in West Nusa Tenggara
E-money transactions will increasingly be a substitute for cash. In Indonesia, QRIS is a popular digital payment system. One way to pay vehicle tax is through QRIS. Adoption of the use of technology as a government step...
The Influence of Learning Capability and Sensing Capability on Digital Entrepreneurship with Market Orientation as Moderation
The Influence of Learning Capability and Sensing Capability on Digital Entrepreneurship with Market Orientation as Moderation. Thesis. Yogyakarta: Faculty of Economics and Business, Yogyakarta State University 2024. Thi...
Knowledge and Practices on Healthcare Waste Management among Healthcare Staff in a Specialized Infectious Disease Hospital in Sri Lanka
Hospital waste management is increasingly becoming important in Sri Lanka since mismanagement may lead severe adverse effect to the patients, health care workers (HCW) as well as to the community. The waste management pr...
Theoretical Study of the Parameters of the Discrete Drum Set Teeth on Fiber Movement
In the article, we will consider a dynamic analysis of the design and parameters of the recommended spinning machine, which provides a uniform supply of fibers to the spinning chamber to obtain high-quality filament in...
The Reality of Creativity in Speaking Skills and Logical Thinking Among Students With Autism Spectrum Disorders Within the English Language Curriculum for Basic Stages in the Light of the List of Multiple Intelligences from the Point of View of English Language Teachers Working in Jordanian Private Education Schools
The current study aimed to identify the reality of creativity in speaking and logical thinking skills among students with autism spectrum disorders within the English language curriculum for basic stages in the light of...