The effect of monosodium glutamate on rat thymocyte proliferation and Bcl-2/bax protein expression
Journal Title: Archives of Medical Science - Year 2006, Vol 2, Issue 4
Abstract
Introduction: Monosodium glutamate (MSG) is one of the commonest food additives in modern nutrition. Recent studies showed the modulatory effect of MSG on rat thymocyte apoptosis in vivo and under in vitro conditions. However, the influence of MSG on thymocyte proliferation is largely unknown. Material and methods: We evaluated the effect of increased MSG concentrations on thymocyte proliferation, as well as the expression of their two apoptosis-related proteins, Bcl-2 and Bax. Proliferation was detected by PCNA monoclonal antibody, and cells were analyzed using a flow cytometer. Expression of Bcl-2 and Bax protein were determined with flow cytometry, using respective monoclonal antibodies. Results: The results obtained in our study demonstrate that MSG significantly decreased thymocyte proliferation, in a dose dependent manner, followed by significantly increased cytotoxicity. The expression of Bcl-2 protein was significantly decreased, while Bax protein expression was not changed in our study. Conclusions: The results presented in our study indicate that MSG significantly modulated intensity of thymocyte proliferation as a consequence of increased cytotoxicity. The expression of Bcl-2 and Bax proteins suggests that this protein level is an important event in thymocyte cytotoxicity, triggered by MSG
Authors and Affiliations
Voja Pavlović, Snežana Cekić
Keywords
Related Articles
- EP ID EP112843
- DOI -
- Views 158
- Downloads 0
Prohepcidin and its possible role in anaemia of pregnancy
Detection of circulating carcinoma cells in peripheral blood collected from patients with ovarian cancer by using different molecular markers – a preliminary report
Introduction: Efficient techniques enabling the ovarian cancer patients’ diagnosis, prognosis and therapy remain far from perfection. Especially, the prognostic significance of circulating tumour cells’ (CTC) presence r...
Uric acid in chronic heart failure: marker or therapeutic target?<br /> Commentary on: Pawan D. Patel, Rohit R. Arora. Uric acid and xanthine oxidase: perspectives in chronic heart failure (Arch Med Sci 2008; 4, 3: 219–225)<br />
Fibroblast growth factors promote pancreatic cell proliferation in normal and STZ – treated hamsters
Introduction: In various organs cell proliferation and differentiation are controlled by growth factors. The knowledge concerning the implication of FGF family members in pancreatic development is still incomplete. Th...
Episodes of severe menorrhagia at menarche in a girl with von Willebrand disease
We describe a case of a 14-year-old girl with von Willebrand disease (vWD) and episodes of severe menorrhagia at menarche, necessitating hospitalization and requiring blood transfusions and administration of purified von...