The effect of oral lead acetate exposure on bax expression and apoptosis index granulose cells antral follicle in female wistar rat (Rattus norvegicus)
Journal Title: Asian Pacific Journal of Reproduction - Year 2017, Vol 6, Issue 2
Abstract
Objective: To prove the effect of administered orally lead acetate exposure on Bax expression and the apoptosis index of granulosa cells on antral follicle female albino rats Wistar strain (Rattus norvegicus). Methods: Post-test only control group, using female albino rats Wistar strain aged 10-12 wk (reproductive age) weighing 100-200 g. Twenty-four animal samples were classified into one control group and three groups exposed to lead acetate in doses of 30, 100, and 300 ppm, respectively. Lead acetate was administered orally through a feeding tube over 30 d. Bax expression of granulosa cells on antral follicle was checked using immunohistochemistry, and apoptosis index were examined using Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). Results: Lead increases apoptosis index on doses of 300 ppm. The increase of the Bax expression granulosa cell on antral follicle was not statistically significant. Conclusion: The exposure of lead acetate administrated orally can increase apoptosis index of granulosa cell on antral follicle although it doesn't affect Bax expression.
Authors and Affiliations
Dian Aby Restanty, Setyawati Soeharto, I Wayan Agung Indrawan Indrawan
Keywords
Related Articles
- EP ID EP240371
- DOI 10.12980/apjr.6.20170202
- Views 209
- Downloads 0
Seasonal variations in serum pregnancy associated glycoproteins during early pregnancy in Aardi goats in central Saudi Arabia
Objective: To accurately detect pregnancy in local Aardi goats of Saudi Arabia before day 30 of pregnancy by using serum caprine pregnancy associated glycoproteins (caPAG) concentrations and to evaluate the effects of he...
Missed estradiol determination resulting in oocyte retrieval and embryo development following controlled ovarian hyperstimulation at early pregnancy: Case report
This paper is a case report on the success of oocyte retrieval and good quality embryo development following controlled ovarian hyperstimulation at early pregnancy. A 30-year-old patient underwent controlled ovarian hype...
Homeostatic relevance of vitamin D in maintaining male fertility in human: Down- regulation of oxidative stress and up-regulation of anti-oxidative defense and steroidal hormones
Objective: To evaluate correlation between the levels of vitamin D and male infertility as well as to determine the efficacy of vitamin D in improving the male fertility by up-regulating the levels of testosterone and sp...
Improvement in cryosurvival of buffalo bull (Bubalus bubalis) sperm by altering freezing rate within critical temperature range
Objective: To optimize the cryopreservation of buffalo bull semen by altering freezing rates within critical temperature range (4 ℃ to -60 ℃ ). Methods: A total of 20 ejaculates each from 5 Murrah buffalo bulls were cr...
Pregnancy outcome of lactating dairy cows assigned for Presynch-Ovsynch synchronization program and inseminated either at detected standing heat or at scheduled fixed time
Objective: To determine if insemination at standing heat results in a similar or higher pregnancy rate compared with fixed time artificial insemination, and to study some factors affecting the pregnancy rate. Methods:...