The Effect of Secretome Hypoxic Mesenchymal Stem Cells on Pro- Inflammatory and Anti-Inflammatory Markers Expression (in Vivo Experimental Study on the Macrophage Cell Polarization of Wistar Male Mice Type 1 DM Model)
Journal Title: International Journal of Multidisciplinary Research and Analysis - Year 2023, Vol 6, Issue 10
Abstract
The autoreactivity of T cells in DMT1 patients causes activation of M1 and M2 macrophages as proinflammatory and anti-inflammatory markers which promote pancreatic cell damage. Secretome Hypoxic Mesenchymal Stem Cells (SH-MSCs) therapy methods play a role in repairing damaged tissue and regenerating pancreatic islet cells. This study aims to examine the effect of SH-MSCs on macrophage cell polarization through the expression of CD68 as a marker of M1 macrophages and CD163 as a marker of M2 macrophages in DMT1 rats. The sample of this study were 5 Wistar rats which were divided into 4 groups; normal group (no treatment), negative control group, and 2 treatment groups with 0.5cc of SH-MSCs (P1 group) and 1cc of SH-MSCs (P2 group). STZ 60 mg/kgBW induced DMT1 in the negative control, P1, and P2 groups. Data were tested parametrically using SPSS 22. The mean of CD68 expression in normal group was 3.06±0.35%, negative control: 21.67±0.93%, P1: 10.98±0.68%, P2: 5.85±0.55%. The mean of CD163 expression in normal group: 22.83±0.51%, negative control group: 3.56±0.50%, P1:15.81±1.80%, P2: 20.80±2.24%. The results of the normality and homogeneity test were p> 0.05. Anova test results p < 0.05. Post Hoc LSD test results p < 0.05. The administration of SH-MSCS was shown to decrease CD68 and increase the CD163 expression significantly in DMT1 rats.
Authors and Affiliations
A’an Haries Pranowo, Danis Pertiwi, Agung Putra
Keywords
Related Articles
- EP ID EP722058
- DOI 10.47191/ijmra/v6-i10-44
- Views 59
- Downloads 0
Financial Reporting Fraud and Models to Assist in Detecting Financial Statement Fraud
The article has used the methodology, analysis and synthesis to accomplish the set research objectives. Accordingly, based on the methodology, the article has presented the concept, purpose and importance of information...
The Impact of ChatGPT on Tourists' Trust and Travel Planning Intention: International Researches and Current Situation in Vietnam
This research investigates the impact of ChatGPT on tourists' trust and travel planning intention, with a focus on international perspectives and the current situation in Vietnam. The study comprises three main sections:...
Community Empowerment-Based Border Tourism Development Strategy in Outermost Archipelago of Talaud Islands Regency
The outermost small islands in the Talaud Island Regency have the potential to be developed into a tourist destination. The purpose of this study is to identify the existing conditions of tourist attraction in the outerm...
Fulfillment of Nutrition Intake of Volleyball Athletes to Achieve Achievement: A Systematic Review
Sport is a genuine work pointed toward working on accomplishing the main introductions for individuals who are individuals or gatherings. He is trusted to acquire knowledge and innovation that is put to good use in order...
Millenial Politeness in Whats’app Application Communication
The technology development change the traditional become the digital. In this digital era, communication take place not only sit side by side, but the presence of smartphone make the distance meaningless. The change fro...