The Effect of Secretome Hypoxic Mesenchymal Stem Cells on Pro- Inflammatory and Anti-Inflammatory Markers Expression (in Vivo Experimental Study on the Macrophage Cell Polarization of Wistar Male Mice Type 1 DM Model)
Journal Title: International Journal of Multidisciplinary Research and Analysis - Year 2023, Vol 6, Issue 10
Abstract
The autoreactivity of T cells in DMT1 patients causes activation of M1 and M2 macrophages as proinflammatory and anti-inflammatory markers which promote pancreatic cell damage. Secretome Hypoxic Mesenchymal Stem Cells (SH-MSCs) therapy methods play a role in repairing damaged tissue and regenerating pancreatic islet cells. This study aims to examine the effect of SH-MSCs on macrophage cell polarization through the expression of CD68 as a marker of M1 macrophages and CD163 as a marker of M2 macrophages in DMT1 rats. The sample of this study were 5 Wistar rats which were divided into 4 groups; normal group (no treatment), negative control group, and 2 treatment groups with 0.5cc of SH-MSCs (P1 group) and 1cc of SH-MSCs (P2 group). STZ 60 mg/kgBW induced DMT1 in the negative control, P1, and P2 groups. Data were tested parametrically using SPSS 22. The mean of CD68 expression in normal group was 3.06±0.35%, negative control: 21.67±0.93%, P1: 10.98±0.68%, P2: 5.85±0.55%. The mean of CD163 expression in normal group: 22.83±0.51%, negative control group: 3.56±0.50%, P1:15.81±1.80%, P2: 20.80±2.24%. The results of the normality and homogeneity test were p> 0.05. Anova test results p < 0.05. Post Hoc LSD test results p < 0.05. The administration of SH-MSCS was shown to decrease CD68 and increase the CD163 expression significantly in DMT1 rats.
Authors and Affiliations
A’an Haries Pranowo, Danis Pertiwi, Agung Putra
Keywords
Related Articles
- EP ID EP722058
- DOI 10.47191/ijmra/v6-i10-44
- Views 64
- Downloads 0
The Role of Sectors in the Socio-Demographic Process of Nukus
: It is natural that all modern small towns move to the level of megacities. The city of Nukus is trying to accept this status on the basis of its socio-demographic process, but it is natural that the bigger the city,...
The Actual Situation of the Insurance Business for Import and Export Goods Transported by the Sea at Insurance Enterprises in Vietnams
To assess the current situation of the insurance business of import and export goods transported by sea at insurance enterprises in Vietnam. The author has used two research methods, namely the methodology and the meta-a...
Determinants Influencing on the Working Motivation of Doctors in Public Hospitals in Hanoi
This research was undertaken to determine the factors affecting the working motivation of doctors in public hospitals in Hanoi. The author conducted a survey and collected 212 valid responses. This research employed quan...
Comparative Study of Cube and Cylinder Crushing Strengths of Laterized Concrete
The non availability or shortage of river sand in some areas affects the concrete construction industry negatively. Hence there is need to find alternatives to river sand and one of the major materials locally available...
Legal Protection of Travelers in Religious Tourism Areas According to Law Number 10 of 2009 Concerning Tourism
Tourism is a sector that contributes to the country's foreign exchange, so tourism's participation in saving the country's economy is needed. saving the country's economy is needed. The tourism development process is ins...