The effect of STP (stimulating tissue preparation) on the phagocytic activity of blood in vaccinated pigs
Journal Title: Бюлетень "Ветеринарна біотехнологія" - Year 2018, Vol 32, Issue
Abstract
Introduction. The effectiveness of preventive vaccinations directly depends on the level of the immune status of the organism. Complex of stress factors associated with the technology of pigs growing, maintenance, care and feeding have negative influence and lead to a decrease of the natural resistance. The relevance of this issue based in its correction using effective means to stimulate factors of natural resistance, including tissue preparations. The goal of the work was to study and carry out comparative analysis of the opsonophagocytic assay (OPA) of vaccinated piglets’ blood with and without previous application of the immunomodulating preparation “STP”. Materials and methods. Studies were carried out on 50 days old piglets, which were divided into control and experimental groups of animals. The piglets of the experimental group were vaccinated against Glasser’s disease with the “Hemofilosan” vaccine along with the application of immunomodulating preparation “STP” to increase the indices of the natural resistance of animals. Results of research and discussion. The results of hematological studies of piglets vaccinated with the Hemofilosan vaccine along with the tissue preparation “STP” confirmed the intense stimulation of the cellular immunity, as the absolute number of lymphocytes increased significantly by 13.7% (p <0.05) compared with this parameters of the control group. OPA showed significant increase in the number of active neutrophils (PA) at the level of 11.8% (p <0.05), phagocytosis number (PN) was significantly higher by 20.0% (p <0.01) and the phagocytosis intensity (PI) increased significantly by 13.2% (p <0.05) compared with the animals of the control group. Since phagocytosis is one of the main mechanisms of natural resistance and an obligatory chain of induction of the immune development, therefore the increasing in the number of active neutrophils, number of phagocytosed cell and phagocytosis intensity indicated the acceleration of the immune response development after application of the tissue preparation “STP” before vaccination. Conclusion and prospects further research. STP application before vaccination of piglets increases the protective phagocytic function of their organism, which is confirmed by the significant increase in PA by 11.8% (p <0.05), PN by 20.0% (p <0.01) and PI by 13 2% (p <0.05), which activated the immune response development against the introduced antigen. Prospects of the researches will be focused on the introduction of the iron and copper nanoparticles to the preparation STP content as biotic microelements to prevent their deficiency in the pigs’ organism and to study of the preparation effect on the indices of natural resistance.
Authors and Affiliations
Svetlana Tiutiun, Olga Gorbatyuk, Valentina Andriyaschuk, Galina Ryzhenko, Tatyana Ukhovska, Alexander Zhovnir
Keywords
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- EP ID EP406285
- DOI 10.31073/vet_biotech32(2)-67
- Views 130
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