The Effect of Telomerase Inhibition on the Expression of Inflammatory Cytokines Affecting the Pathogenesis of Multiple Myeloma Cell Line U266
Journal Title: مجله علمی- پژوهشی دانشگاه علوم پزشکی قم - Year 2018, Vol 12, Issue 4
Abstract
Background and Objectives: Telomerase is an enzyme, which is overexpressed in 80-90% of cancers. Simultaneous activities of telomerase and NF-κB are required for progression of many cancers. In recent years, researchers have found out a close relationship between telomerase and the transcription factor NF-κB. Increased expression of telomerase is associated with significant increases in the expression level of NF-κB and endogenous genes, such as IL-6 and TNF-α. In recent years, several methods have been proposed to inhibit telomerase in cancer cells. Therefore, If it is possible to inhibit telomerase activity and consequently reduce the expression of inflammatory cytokines, the NF-κB signaling pathway, and the expression of target genes in the multiple myeloma disease. In this study, the effect of MST-312 (a derivative of green tea) with telomerase inhibition activity, was investigated on the treated U266 cell line and the expression of inflammatory cytokines. Methods: In this experimental study, U266 cells, were treated with different dosed of MST-312 for 48 hours, and cellular apoptosis, was assessed by Annexin V Apoptosis Detection Kit. Then, to assess the expression of IL-6 and TNF-α genes, cells were treated with MST-312 (2μM for 48 hours) and the RNA of these cells, was extracted. In the following, real-time PCR method was used to investigate gene expression level. Results: In this study, an increase in apoptosis and a decrease in the expression of IL-6 and TNF-α genes in U266 cells, was observed after 48 hours of exposure with 2μM MST-312. In addition, no cytotoxic effect was observed on normal blood mononuclear cells. Conclusion: The results of the present study indicated that inhibition of telomerase activity by MST-312, can be considered as a novel treatment strategy for multiple myeloma.
Authors and Affiliations
Saeede GHiyasi, Zahra Ameri, Gholamhossein Hassanshahi, Roohollah Mirzaee, Mohsen Ehsan, Noushin Pouryazdanpanah, SHima Kazemzadeh, Ahmad Fatemi
Keywords
Related Articles
- EP ID EP479630
- DOI 10.29252/qums.12.4.1
- Views 52
- Downloads 0
تأثیر سایتوتوکسیک تستوسترون بر رده سلولی سرطان معده و ارزیابی فعالیت کاسپازهای 3، 8 و 9
زمینه و هدف: مطالعات نشان داده‌اند استروئیدهای جنسی بر تکثیر سلول‌های سرطانی در سطح سلولی و مولکولی تأثیرگذارند. در این مطالعه به بررسی اثرات ضد تکثیری تستوسترون بر رده سلولی سرطان معده و ارزیابی فعالیت کاسپازهای 3، 8 و...
Comparison of Susceptibility Testing of E-test Strips with Cefoxitin and Oxacillin Disks in Identification of Methicillin-resistant Staphylococcus saprophyticus Strains
Background and Objectives: Over the years, Staphylococcus saprophyticus, in addition to becoming a serious pathogen, has become resistant to a wide range of antibiotics. Offering an inexpensive, rapid, and available meth...
ارتباط نمایه توده‌بدنی و دور کمر با عزتنفس در مردان جوان ایرانی
زمینه و هدف: امروزه، ملاک‌های پذیرش اجتماعی و موفقیت تغییر کرده که بر سلامت روان‌شناختی ازجمله عزتنفس افراد نیز تأثیر‌گذار است. از طرفی در دهه‌های اخیر، چاقی و اضافه وزن رو به افزایش بوده که می‌تواند تأثیرات زیادی بر...
اثر لیپوپپتیدهای حلقوی باسیلوس آتروفئوس (سویه HNSQJYH170) بر گونه‌های کاندیدا
زمینه و هدف: در طول سال های اخیر، عوامل متعددی مانند طیف وسیعی از آنتی بیوتیک ها، بیماران مبتلا به نقص ایمنی، شیمی درمانی سیتوتوکسیک و پیوندها خطر ابتلا به عفونت های قارچی را در انسان ها، افزایش داده است. در میان تمام پا...
Antibacterial Activity of Rumex cyprius Seeds Against Escherichia coli and Staphylococcus aureus
Background and Objectives: The infections caused by Escherichia coli and Staphylococcus aureus resistant strains often originate from hospitals, and their prevalence is increasing worldwide. Therefore, many efforts are b...