THE EFFECT PECULIARITIES OF SINGLE DOSE LOCAL GAMMA-IRRADIATION OF RATS ON THEIR REPRODUCTIVE SYSTEM AND SPERMATOGENESIS
Journal Title: Scientific journal «Biological Systems: Theory and Innovation» - Year 2017, Vol 2, Issue 270
Abstract
Male reproductive system and germinal cells are very sensitive to different xenobiotics, especially to ionizing radiation, which is known to damage biological membranes, elements of cytoskeleton and DNA, whereby showing up in chromosomal and genic mutations on genome level. The impact of local gamma-irradiation by the dose 2,0Gy and 5,0Gy, respectively, of the lower quarter of animal body on further rat development, sexual organs and accessory glands growth, normal and abnormal sperm production was to be studied. Laboratory white rats in the age of 10 months were irradiated by gamma rays of 60 Co by two separate doses 2,0 and 5,0Gy, respectively. Epididymal sperm quantity, motility and viability was analyzed. Statistical analysis was carried out by «ANOVA» and unpaired Student’s test with Bonferoni amendment. The gamma-irradiation was shown to have no influence on animal growth. Meanwhile the two radiation doses induced the suppression of testicular and ventral prostate development in the first 4-6 months after irradiation, whereas the epididymices were negatively affected only by 5,0Gy. The dose of 2,0Gy exerted the hormesis effect on the epididymices weight. The radiation suppressive action on sexual organs and accessory glands was characterized by its temporary manifestation. Thereafter testicular parenchyma and spermatogenesis started to resume accompanying by an elevation in the amount of viable and morphologically normal spermatozoa. In view of this, in the late post-radiation period the weight of testicles and ventral prostate tended to attain control values. The experiments performed have not established any differences in the body weight means between control and gamma-irradiated rats over 1 year period. The mean weight of testicles after irradiation by 2,0 and 5,0Gy primarily decreased and then gradually restored up to control level. The hormesis effect of radiation on epididymal weight dealt with the acceleration of sperm production through mobilization of stem spermatogonia inert pool and its rapid proliferation. Despite spermatozoid overproduction at 2,0Gy the quantity of viable and morphologically normal spermatozoa in this case abated and was lower than in control
Authors and Affiliations
L. Grubska, I. Gudkov, A. Klepko, O. Trofimenko
Keywords
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