THE EFFECTS NANO-SILVER ON CONTAMINATION OF SPRING BARLEY ‘EUNOVA’ IN VITRO
Journal Title: Folia Pomeranae Universitatis Technologiae Stetinensis Agricultura Alimentaria Piscaria et Zootechnica - Year 2017, Vol 44, Issue 338
Abstract
One of the available methods to control in vitro contaminations is nano-silver. Nanosilver is a non-toxic material which shows high capabilities in eliminating microorganisms, e.g., bacteria, viruses or fungus. The aim of the current study was to evaluate the potential of nano- -silver particles on decontamination, growth parameters (plant length, root length) and leaves colour of spring barley ‘Eunova’ in in vitro culture. Plant material consisted of embryos of barley which were placed on MS medium supplemented with 4.0 and 6.0 0 mg · dm–3 of nano-silver or submerged in 50 or 100 0 mg · dm–3 of nano-silver for 2 or 4 h. The control consisted of embryos treated with 70% alcohol. After 28 days it was observed, that both of the disinfection methods, operating time as well as concentrations of nano-silver, had an influence on a number of contaminations, shoot and root length and colour of the barley leaves. The smallest efficiency (100% contaminations) was found after embryos submerged in 50 mg dm-3 of nano-silver for 2 h. On the other hand, the best disinfection efficiency (0% infection) was obtained when 4.0 0 mg · dm–3 nano-silver was added to MS medium. Barley embryos soaked in 100 mg · dm–3 in nano-silver solution and grown on MS media with addition of nano-silver independent of their concentration showed similar disinfection effectiveness.
Authors and Affiliations
Marcelina KRUPA-MAŁKIEWICZ, Ireneusz OCHMIAN, Kamila MIJOWSKA, Krzysztof CENDROWSKI
Keywords
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- EP ID EP410415
- DOI 10.21005/AAPZ2017.44.4.08
- Views 76
- Downloads 0
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