The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells
Journal Title: Bezmiâlem Science - Year 2019, Vol 7, Issue 2
Abstract
Objective: Green tea (Camellia sinensis) is one of the most consumed beverages in the world. C. sinensis consumption may prevent tumor and other diseases. Insulinomas are pancreatic islet cell tumors. In present study, the effects of the extract from C. sinensis were investigated in rat insulinoma INS-1 cells. Methods: C. sinensis leaves were collected in Rize, Turkey. The leaves were brewed in tap water for 20 and 40 minutes at 80°C. INS-1 cells were treated with different doses (1-5 mg/mL) of C. sinensis extract for 24 h. Cell viability, proliferation, death were examined. The malondialdehyde, glutathione and protein carbonyl levels were measured in INS-1 cells given C. sinensis extract. Results: The cell viability and the percentage of proliferating cell nuclear antigen immunopositive cells decreased, while the percentage of TUNEL positive cells increased in INS-1 cells treated with the extract from C. sinensis according to control group. While malondialdehyde and protein carbonyl levels in INS-1 cells treated with C. sinensis extract decreased, glutathione levels increased according to control group. Conclusion: The results indicate that the extracts from C. sinensis inhibited proliferation and caused apoptosis in INS-1 cell. C. sinensis may be natural agent for supporting the treatment of pancreatic tumors.
Authors and Affiliations
Ayşe Karatuğ KAÇAR, Melike ERSÖZ, Işıl SEZEKLER, Zeynep Mine COŞKUN
Keywords
Related Articles
- EP ID EP49057
- DOI 10.14235/bas.galenos.2018.2287
- Views 237
- Downloads 0
Pseudohypoparathyroidism Type Ia with Normocalcemia
Pseudohypoparathyroidism (PHP) is a heterogeneous group of disorder with parathormone target organ resistance, characterized by hypocalcemia, hyperphosphatemia and high blood parathormone (PTH). Typical phenotypic sympto...
Spina Iliaca Anterior Superior Avulsion Fracture: A Case Report and Review of the Literature
Spina iliaca anterior superior (SIAS) avulsion fracture is a type of injury that is rare and possible to be overlooked in emergency services. SIAS is the insertion area of the Tensor fascia lata and Sartorius muscles, wh...
Comparison of the Role of Endocan With Other Laboratory Tests in Diagnosis of Acute and Perforated Appendicitis in Children
Objective: In this study, we aimed to determine the role and efficacy of endocan and other routine laboratory tests to diagnose of acute appendicitis (AA) and determining perforation. Methods: One hundred patients under...
The Inhibitory Effect of Ileal Mucosal Media Originated from FVB/N Mice Strain on Escherichia coli LF82 Invasion
Objective: The aim of this study was to investigate the effect of healthy mucosa on adhesive and invasive properties of AIEC reference strain Escherichia coli (E. coli) LF82. For this purpose, we had designed special med...
Extra-Parenchymal Chest HRCT Findings in Patients with Systemic Sclerosis at the Time of Initial Diagnosis
Objective: In systemic sclerosis (SS) patients who are receiving treatment, findings of extra-parenchymal chest involvement (such as thymic abnormality, mediastinal lymph node enlargement) have been previously examined....