THE EFFECTS OF CISPLATIN ON EMBRIONIC RENAL CELL (HEK-293) AMINO ACID METABOLISM
Journal Title: International Journal of Current Medical and Biological Sciences - Year 2022, Vol 2, Issue 2
Abstract
Background:Cisplatin is one of the most widely used and most potent chemotherapy drug, due to its side effects in normal tissues and organs, especially its nephrotoxic effects in the kidneys, its use was limited. Therefore, new studies are being conducted to elucidate the mechanism of nephrotoxicity and to identify new protective agents in order to reduce the side effects of cisplatin. This study was conducted to investigate the effects of cisplatin on amino acid metabolism of embryonic kidney cells (HEK-293).Materials and Methods: In the study, HEK-293 were incubated with cisplatin at different doses (1-20μg) in appropriate medium for 24 hours, and then the cells were homogenized. The intracellular free amino acid profile in the samples was analyzed by LC-MS/MS method.Results: Compared to the control group, a significant decrease was observed in 1-methylhistidine, 3-methylhistidine, aspartic acid and cystine levels, while a significant increase was found in other amino acids. As a result of conducting advanced bioinformatic analyzes through Principal Component Analysis (PCA) and Variable Importance in Projection (VIP), it was determined that the most significant difference that was realized between the control and cisplatin applied groups was in glutamine, glutamic acid, glycine, arginine and lysine amino acids. Conclusions: It was observed that high doses of cisplatin can inhibit protein synthesis in kidney cells and increase the amount of free amino acids by increasing the digestion of intracellular damaged proteins.
Authors and Affiliations
ERKAN ARSLAN, Ismail koyuncu, Sukru Akmese
Keywords
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