THE EFFECTS OF MEDIA COMPOSITION ON IN VITRO GYNOGENIC EMBRYO INDUCTION IN Allium tuncelianum (KOLLMAN) OZHATAY, MATTHEW, SIRANECI)
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 58
Abstract
Allium tuncelianum is utilized as garlic in Eastern region of Turkey. There have been conducted out some studies for propagation of Allium tuncelianum but there is not any breeding material yet. It is the first research about determining of gynogenic induction capacity of Allium tuncelianum (Kollman) Özhatay, Matthew, Şiraneci) by using flower bud culture. To determined effect of plant growth regulators on gynogenesis induction, 1 mg/l and 2 mg/l of 2,4-D and BAP and their combinations were supplemented in BDS medium was. Induction medium including different combinations of auxin (2,4-D) and cytokinin (BAP) were effective on callus development on explants. The highest callus formation rates were obtained from 2+1 mg/l 2,4-D+BAP and 2+2 mg/l 2,4-D+BAP combinations. It was provided the development of the flower buds in induction medium and callus has formed at 55,28% of flower buds but plantlets could not be achieved from callus. Therefore, it is thought to be more obscurity on gynogenesis induction of Allium tuncelianum. Haploid plant could not be obtained in this research but this study is important due to the guidance for future studies.
Authors and Affiliations
FAIKA YARALI
Keywords
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