The establishment of a high-resolution melting curve genotyping method for ABCG2*376T allele of Jr(a-) and its distribution frequency study
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 7
Abstract
Objective To establish a high-throughput detection method for ABCG2*376T allele of Jr(a-), and apply it to the study of the frequency of this allele in the Chinese population. Methods The specific primers were designed and synthesized, the sample carrying homozygous ABCG2*376T alleles, obtained in the previous study, was used as the homozygous positive control, and the sample carrying heterozygous allele as the heterozygous positive control. The wild-type sample was used as a negative control, and a high-resolution melting curve(HRM) method for detecting this allele was established. The established method was used to screen DNA samples from blood donors in Guangzhou, and the samples carrying ABCG2*376T alleles were sequenced to confirm the accuracy of the HRM method. Results A HRM method, which can detect ABCG2*376T allele and accurately type homozygotes and heterozygotes at the same time, had been established successfully. Fifteen individuals with heterozygous alleles were screened out of 1 560 blood donors in Guangzhou, while none homozygous allele was detected. Conclusion The HRM method can be used to accurately screen and type ABCG2*376T allele. The frequency of this allele in Chinese population is about 0.48%(15/3120).
Authors and Affiliations
Zhen WANG, Shuangshuang JIA, Zhijian LIAO, Chunyan MO, Ling WEI, Runqing ZHANG, Guangpin LUO, Yanli JI
Keywords
Related Articles
- EP ID EP738282
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.07.002
- Views 8
- Downloads 0
Preliminary identification of CD4 T cell epitopes in genotype 1 and 6 of hepatitis C virus
Objective To study the CD4 T cell epitopes in Core and NS3 protein of genotype 1(GT1) and 6(GT6) of hepatitis C virus(HCV). Methods A total of 298 overlapping peptides(16-mer) spanning Core and NS3 protein of GT1 and GT6...
Evaluation of the efficacy and safety of human coagulation factor Ⅷ in the treatment of hemophilia A patients
Objective To evaluate the efficacy and safety of human coagulation factor Ⅷ developed by Shenzhen Weiguang Biological products Co, Ltd in the treatment of patients with hemophilia A. Methods A prospective, multi-center,...
Retrospection and multivariate Logistic regression analysis of unqualified blood testing among voluntary blood donors in Guangzhou
Objective To retrospectively analyze the unqualified blood screening results of voluntary blood donors in Guangzhou area during 2016~2020, and analyze various factors that affect the blood screening results, for the purp...
Distribution characteristics of unexpected antibody in 1 779 patients and corresponding blood transfusion strategy
Objective To observe the distribution of the unexpected antibodies in order to study the safety and strategies in 1 779 cases of clinical blood transfusion. Methods A total of 1 779 patients with unexpected antibodies we...
Bioactive ingredients in activated platelet-rich plasma derived from cord blood and adult blood via Luminex technology: a comparative study
Objective To compare the bioactive ingredients in activated platelet-rich plasma (PRP) from cord blood and adult blood, explore its reasonable indicators reflecting the inflammatory regulation ability, in order to guide...