THE EVALUATION OF TOPOGRAPHY BORRELIA BURGDORFERI BY ATOMIC FORCE MICROSCOPE (AFM)
Journal Title: Health Problems of Civilization - Year 2015, Vol 9, Issue 3
Abstract
The aim of the present work was to evaluate the topography of spirochetes' cells Borrelia burgdorferi s.s. B31 in atomic force microscope [AFM]. Results: The length of spirochetes B. burgdorferi has ranged between 15.38-22.68pm. The cells of spirochetes do not constitute structures of a fixed diameter and height. Thus, in order to identify real parameters of cells, the horizontal distance and vertical distance have been used in the measurements. The average value of a spirochetes’ diameter has been estimated by taking series of measures and it is 0.40 pm. The average value of a spirochetes’ height has been estimated by taking series of measures and it is 70.14 nm. The analysis of a relation between measured parameters of spirochetes: diameter and height revealed that along with the growth of diameter of a bacteria cell, its height also grows. The average value of a fibers’ diameter has been estimated by taking series of measurements and it is 0.09 um and the average height of fibers was 7.91 nm. Conclusions: The atomic force microscope (AFM) is a modern tool with a broad spectrum of observatory and measure abilities and is a technique which has been used in biology and microbiology to investigate the topography of surface and in the evaluation properties of cells.
Authors and Affiliations
Malgorzata Tokarska-Rodak, Maria Koziol-Montewka
Keywords
Related Articles
- EP ID EP218942
- DOI 10.5114/hpc.2015.57127
- Views 67
- Downloads 0
Knowledge of coronary arteriosclerosis risk factors and their occurrence and the lifestyles of the first-year medical students
Background Coronary arteriosclerosis risk factors increase the probability of the coronary heart disease and accompanying complications to a significant extent, acting independently from other circumstances. Material an...
Life satisfaction in women after mastectomy – a pilot study
Background Cancer is the second most common cause of morbidity and mortality in Poland and breast cancer is the most frequent malignant neoplasm occurring in Polish women. The aim of this paper is to analyse life satisfa...
TICK-BORNE BACTERIAL DISEASES IN POLAND
Many infectious diseases are spread by a vector. Some microorganisms require both the vertebrate as well invertebrate host to complete their life cycle. In this way, many bacterial diseases are transmitted to humans. Wit...
EFFICIENCY OF BIOFLAVONOID QUERCETIN AND RNA-CONTAINING DRUG SODIUM NUCLEINAT IN COMPLEX TREATMENT OF PATIENTS WITH MYOCARDIAL INFARCTION AND FUNCTIONAL LIVER DISORDERS
<B>Background</B>. The study has shown that functional liver disorders develop in patients with MI, which requires correction. <B>Material and methods</B>. The study involved 149 patients with acute MI. All examinations...
The role of reactive oxygen spesies in the pathogenesis of experimental maxillary sinusitis
Simulated maxillary sinusitis was observed in guinea pigs following the surgical incision in superior cervical sympathetic ganglion. Additionally, toxic hepatitis development was stimulated after exposure to tetrachlorme...