The Fallacy of Identification by Neutralization in the Light of Non-Cytopathic Effect Producing Enterovirus Strains
Journal Title: Microbiology Research Journal International - Year 2017, Vol 22, Issue 4
Abstract
We describe the characterization of an enterovirus isolate recovered from untreated raw sewage in Ibadan, southwest Nigeria in 2010. The isolate was neutralized by specific antisera and consequently identified as Echovirus 7 (E7). Subsequent molecular characterization showed the isolate to be a mixture of E7 and Coxsackievirus A24 (CV-A24) thereby suggesting the CV-A24 strain to be non-cytopathic effect producing. It is therefore crucial that identities of enterovirus strains determined by neutralization assay be verified by molecular characterization to ensure they do not have any non-cytopathic effect producing strain(s) lurking unnoticed.
Authors and Affiliations
Moses Olubusuyi Adewumi, Temitope Oluwasegun Cephas Faleye, Johnson Adekunle Adeniji
Keywords
Related Articles
- EP ID EP316212
- DOI 10.9734/MRJI/2017/38280
- Views 75
- Downloads 0
Smokeless Tobacco – Ammunition against Dental Caries? A Microbial Study
Aims: The tobacco plant, Nicotiana tabacum, has been one of the major risk factors for oral cancer. However, the literature has endowed it as “holy herb” since the pre-Columbian era as being used for treating pain, poiso...
Isolation and Identification of Some Enterobacteria from Retailed Convenience Foods
Meat pie and Fish roll samples from five different local government areas (LGAs) in Ibadan, Nigeria were analysed microbiologically for presence of Enterobacteriaceae and other common food borne pathogens. Sampling was d...
Antimicrobial Properties of Ocimum sanctum and Calotropis gigantea Leaves
Objective: To evaluate antimicrobial and phytochemical activity of hexane and ethanolic extract of Ocimum sanctum and Calotropis gigantea leaves on clinical pathogens. Materials and Methods: The agar diffusion test was u...
Prevalence of Non-Typhoidal Salmonella among HIV/AIDS Patients and Poultry Chicken in Ekiti State
Salmonella is an important cause of infection in both humans and animals. This study was therefore carried out to identify the non-typhoidal Salmonella isolates from poultry chicken which is one of the main animal reserv...
Hospital Antibiogram: A Necessity in Monitoring Sensitivity of Isolates and Rationale Use of Antibiotics’
Background: The laid protocols by CDC (center for disease control) narrated the dire need of local anti-biograms. Therefore the current study had been planned to acquire the knowledge about sensitivity pattern of various...