The hematological malignancies related to primary hypereosinophilia and their diagnostics
Journal Title: Advances in Hygiene and Experimental Medicine - Year 2014, Vol 68, Issue
Abstract
Published in 2008, by experts of the World Health Organization, the new classification of hematological malignancies forced a change of look at chromosomal aberrations and gene mutations, which are important in establishing the diagnosis and prognosis for patients with these malignancies. The new classification includes a new category of neoplasms – hematological malignancies with hypereosinophilia. Due to the high diversity of causes of hypereosinophilia and underlying genetic changes, their differential diagnosis is based on classical cytogenetics, fluorescence in situ hybridization (FISH) and genetic molecular techniques. Cytogenetic analysis of bone marrow cells showed that the majority of hypereosinophilia cases can be characterized by the presence of normal karyotype. Therefore, routine cytogenetic diagnostics should be complemented by FISH with break-apart probes for potentially rearranged genes (e.g. CBFB, ETV6) and unique probes for fusion genes (e.g. FIP1L1-PDGFRA), specific for hypereosinophilia-associated diseases. In differential diagnosis of hypereosinophilia, the analysis of characteristic gene mutations (e.g. cKIT) and gene fusions (e.g. ETV6-PDGFRB) is also applied, using molecular genetic methods.
Authors and Affiliations
Katarzyna Skonieczka, Karolina Matiakowska, Olga Haus
Keywords
Related Articles
- EP ID EP67567
- DOI -
- Views 137
- Downloads 0
Selected indices of peritoneal fibrosis in patients undergoing peritoneal dialysis
Peritoneal dialysis is an alternative to hemodialysis in the treatment of patients with end-stage renal disease. Long-term use of peritoneal dialysis is limited by progressive alterations in the peritoneal membrane. The...
Rola nauk biologicznych w zrozumieniu genezy i nowego podejścia terapeutycznego do choroby Alzheimera
W pracy porównano historyczny obraz przyczyn choroby Alzheimera (AD) ze współczesnym spojrzeniem na czynniki, które mogą leżeć u podstaw jej symptomów. Nauki biologiczne zajmujące się budową i fizjologią komórki pozwol...
Procalcitonin (PCT), contemporary indicator of infection and inflammation
Procalcitonin is a protein synthesized during sepsis and inflammation. In these states, its production is stimulated by inflammatory mediators and bacterial toxins. Routine determination of PCT concentration is useful in...
Treatment of renal ANCA-associated vasculitides
Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitides (AAV) are a group of small vessel vasculitides which commonly affect the kidneys, manifesting as rapidly progressive glomerulonephritis. In this review,...
Cancer stem cells
Cancer stem cell theory gains increasingly greater significance in the world of medicine. Numerous findings of scientific research in vivo and in vitro indicate that it is the population of undifferentiated, self-renewin...