The Impact of Calcium Hydroxide on the Osteoinductive Capacity of Demineralized Freeze-Dried Bone Allograft: an In-Vitro Study
Journal Title: Journal of Rehabilitation Sciences and Research - Year 2018, Vol 19, Issue 1
Abstract
Statement of the Problem: A great challenge in periodontal therapy is the regeneration enhancement of osseous defects through applying osteoinductive materials. Demineralized freeze-dried bone allograft (DFDBA) has already been introduced as an allograft with osteoconductive and variable osteoinductive properties. Calcium hydroxide [Ca(OH)2] is an available well-known material in dentistry, which induces hard tissue formation. Purpose: This study evaluated the efficiency of combination of DFDBA and Ca(OH)2 in improving the quality of osteoinduction of DFDBA. Materials and Method: Human bone marrow-derived mesenchymal stem cells were taken from volunteers’ iliac crest. Cell proliferation was determined by MTT test at 18, 24 and 48 hours post-culture in 10 groups. The employed material were 0.5, 1.0 mg/ml Ca(OH)2 in two forms of suspension and pH-adjusted solution, 10mg/ml DFDBA per se and in combination with 0.5 and 1.0 mg/ml Ca(OH)2. Mineralization was assessed by Alizarin red staining in 10 mg/ml DFDBA, DFDBA+ 0.5 and 1 mg/ml Ca(OH)2 in solution and suspension forms. The data were statistically analyzed by using one-way ANOVA and Tukey’s post-hoc test (p< 0.05). Results: The pH-adjusted solutions exhibited better cell proliferation compared with the suspension groups. The combination of 0.5mg/ml Ca(OH)2 solution and DFDBA increased the cell proliferation and mineralization compared with DFDBA per se (p= 0.033). Conclusion: The combination of Ca(OH)2 with DFDBA improved the osteoinductivity of DFDBA.
Authors and Affiliations
Hengameh Khosropanah, Nazila Lashkarizadeh, Maryam Ayatollahi, Zohreh Mostafavipour
Keywords
Related Articles
- EP ID EP266239
- DOI -
- Views 90
- Downloads 0
Effects of Universal and Conventional MDP Primers on the Shear Bond Strength of Zirconia Ceramic and Nanofilled Composite Resin
Statement of the Problem: The clinical success of ceramic depends on the quality of the bond between the zirconia and resin cement. Purpose: In the present study, the effects of universal and conventional MDP-containing...
The Grammatical Deficits of Persian-Speaking Children with Specific Language Impairment
Background: Specific language impairment (SLI) is one of the most common disorders of language development in children, which has been less addressed in Persian studies. The aim of this study was to evaluate the grammati...
Prediction of Scapular Dyskinesis Through Electromyographic Indices of Scapulothoracic Muscles in Female Overhead Athletes
Background: Shoulder joint function and the athletic performance of overhead athletes are influenced by scapular dyskinesis (SD) due to its high prevalence among these athletes and the motor interaction between the scapu...
Diagnostic Efficacy of Deeper Sections in Routine Oral Histopathology Practice: a Retrospective Study
Statement of the Problem: Histopathology is a gold standard diagnostic tool for oral lesions. Nonspecific and overlapping microscopic features often pose diagnostic challenges in routine practice. In such conditions, dee...
Relationship between Quality of Life and Hypertonia in Young Adults with Spastic Cerebral Palsy
Background: The complications associated with spastic cerebral palsy can affect the physical ability and quality of life of individuals suffering from this motor disorder. The aim of this study was to assess the relation...