The Impact of Calcium Hydroxide on the Osteoinductive Capacity of Demineralized Freeze-Dried Bone Allograft: an In-Vitro Study
Journal Title: Journal of Dentistry, Shiraz University of Medical Sciences - Year 2018, Vol 19, Issue 1
Abstract
Statement of the Problem: A great challenge in periodontal therapy is the regeneration enhancement of osseous defects through applying osteoinductive materials. Demineralized freeze-dried bone allograft (DFDBA) has already been introduced as an allograft with osteoconductive and variable osteoinductive properties. Calcium hydroxide [Ca(OH)2] is an available well-known material in dentistry, which induces hard tissue formation. Purpose: This study evaluated the efficiency of combination of DFDBA and Ca(OH)2 in improving the quality of osteoinduction of DFDBA. Materials and Method: Human bone marrow-derived mesenchymal stem cells were taken from volunteers’ iliac crest. Cell proliferation was determined by MTT test at 18, 24 and 48 hours post-culture in 10 groups. The employed material were 0.5, 1.0 mg/ml Ca(OH)2 in two forms of suspension and pH-adjusted solution, 10mg/ml DFDBA per se and in combination with 0.5 and 1.0 mg/ml Ca(OH)2. Mineralization was assessed by Alizarin red staining in 10 mg/ml DFDBA, DFDBA+ 0.5 and 1 mg/ml Ca(OH)2 in solution and suspension forms. The data were statistically analyzed by using one-way ANOVA and Tukey’s post-hoc test (p< 0.05). Results: The pH-adjusted solutions exhibited better cell proliferation compared with the suspension groups. The combination of 0.5mg/ml Ca(OH)2 solution and DFDBA increased the cell proliferation and mineralization compared with DFDBA per se (p= 0.033). Conclusion: The combination of Ca(OH)2 with DFDBA improved the osteoinductivity of DFDBA.
Authors and Affiliations
Hengameh Khosropanah, Nazila Lashkarizadeh, Maryam Ayatollahi, Zohreh Mostafavipour
Keywords
Related Articles
- EP ID EP303059
- DOI -
- Views 89
- Downloads 0
The Effect of Incorporation of 0.5 %wt. Silica Nanoparticles on the Micro Shear Bond Strength of a Resin Modified Glass Ionomer Cement
Statement of the Problem: The clinical success of glass ionomer restorations depends on the strength of resin-modified glass ionomer (RMGI) cement bonding to dentin and there is limited information available regarding th...
Diagnosis of Approximal Caries after Delayed Scanning of Photostimulable Phosphor Plates
Statement of the Problem: Considering the high diagnostic accuracy and wide dynamic range of photostimulable phosphor plates (PSPs), they can be a good alternative for radiographic films. Purpose: This study was aimed to...
A Rare Clinical Presentation of an Osteochondroma of Coronoid Process of Mandible
Osteochondroma (osteocartilaginous exostosis) is one of the most common benign tumors of bone but is rare in the craniofacial region. Only a few cases of osteochondroma of the coronoid process have been reported in the l...
Endodontic Management of Calcified Oehler’s Type IIIb Dens Invaginatus in Permanent Maxillary Lateral Incisor Using Cone Beam Computed Tomography
Dens Invaginatus is a developmental disturbance resulting from abnormal morpho differentiation. It is characterized by an invagination of the enamel and dentin, creating a lumen inside the affected tooth, which may exten...
Evaluation of the Effects of Three Plant Species (Myrtus Communis L., Camellia Sinensis L., Zataria Multiflora Boiss.) on the Healing Process of Intraoral Ulcers in Rats
Statement of the Problem: Use of traditional medicine to relieve human sufferings has a very long history. The effects of these plants in wound curing and subsequently making the best mucosa patch for treatment of oral u...