THE IMPACT OF CARBON , NITROGEN SOURCES AND METALSALTS ON PRODUCTIVITY OF CAROTENE SYNTHESIZING STRAINS BACILLUS SUBTILIS 1.1 AND B. AMYLOLIQUEFACIENS UCM B-5113
Journal Title: Мікробіологія і біотехнологія - Year 2015, Vol 0, Issue 1
Abstract
Puspose. To investigate the impact of carbon, nitrogen sources and metal salts on the productivity of carotene synthesizing strains B. amyloliquefaciens UCM B-5113 and B. subtilis 1.1. Methods. The features of growth and pigment production of investigated strains were determined by submerged cultivation in periodic conditions based on biotechnology parameters. The pigments extraction was carried out with the use of mixture of chloroform and methanol (2:1). The qualitative composition of carotenoids was assessed by their absorption spectra. Results. The highest total carotenoids productivity of strains B. amyloliquefaciens UCM B-5113 and B. subtilis 1.1 was on the media containing arabinose, maltose, (NH4)2SO4 and FeSO4 and composed 168 and 384 mg/l, 310 and 570 mg/l, 296 and 640 mg/l, 309 and 605 mg/l, respectively. Conclusion. Arabinose, maltose, (NH4)2SO4 and FeSO4 were the optimum sources of carbon, nitrogen and metal salts for growth and carotenoids synthesis by investigated Bacillus strains. Overall productivity of strains B. subtilis 1.1 and B. amyloliquefaciens UCM B-5113 was higher than on control medium at 2.1–3.9, and 1.6–2.7 times, respectively, with constant quality of pigment composition.
Authors and Affiliations
O. Nechypurenko, M. Kharhota, L. Avdeeva
Keywords
Related Articles
- EP ID EP376609
- DOI 10.18524/2307-4663.2015.1(29).48039
- Views 107
- Downloads 0
ELECTRORETENTION AS MOTIVE FORCES OF CONCERTED ACTION OF ENZYMES ON THE MEMBRANES OF LIVING CELLS
Visual (by light microscope) observation of the phenomenon of the electroretention of microbial cells and the experiments with immobilization of the enzymes on the collectors in electric field suggests a decisive role of...
INFLUENCE OF POTASSIUM ALUM ON THE SYNTHESIS OF MICROBIAL SURFACE-ACTIVE COMPOUNDS
Aim. The investigation of stimulating effect of potassium alum on biosurfactant synthesis by bacteria of different taxonomic groups. Methods. Bacterial strains were cultivated on nutrient media with the addition of alum....
REMOVAL OF PHENOL FROM WATER BY BACILLUS SUBTILIS ONU551 BACTERIA, ADHESIZED ON THE CARRIERS OF DIFFERENT NATURE
Aim. Investigation of the efficiency of removal phenol from water by bacteria Bacillus subtilis ONU551 adhered on the carriers of different nature. Methods. The concentration of phenol in water was determined by extracti...
ANTIMICROBIAL PROPERTIES OF MYCELIUM AND BASIDIOCARPS OF GANODERMA LUCIDUM (СURTIS) P. KARST.
Aim. Evaluation of the reishi mushroom, Ganoderma lucidum, vegetative mycelium and water extracts of basidiocarps influence on the growth of opportunistic microorganisms. Materials and methods. Antimicrobial activity of...
SENSITIVITY TO UV-C OF GRAMNEGATIVE EPIPHYTIC BACTERIA FROM CHERNOBYL EXCLUSION ZONE
Aim: to study the sensitivity to UV of the bacterial genera Pseudomonas and Pantoea from the epiphyte of plant samples from 10 km of Chernobyl Exclusion Zone (CEZ). Methods: The objects of the study were the strains of b...