The in vitro Potential Oncolytic Effect of Lentogenic and Velogenic Newcastle Disease Viruses on MCF-7 and Caco-2 Cell Lines Compared to Chemotherapies
Journal Title: Journal of Applied Veterinary Sciences - Year 2024, Vol 9, Issue 1
Abstract
Cancer is the leading cause of death worldwide, with breast and colorectal cancers being the two most common cancer forms.The present work was designed to investigate the probable oncolytic effect of lentogenic and velogenic Newcastle disease viruses on MCF-7 and Caco-2 cell lines compared to the commonly used chemotherapies as an in vitro preliminary study to further prelude an in vivo study.The cytotoxic effects ofNewcastle disease virus strains NDV/chicken/Egypt/Giza/2015 (velogenic NDV genotype VIID) and Lasota strains, as well as the commonly used chemotherapies (Paclitaxel or Doxorubicin) were investigated on MCF-7 and Caco-2 cell lines at different concentrations. Both the human colorectal adenocarcinoma (Caco-2) and the Michigan Cancer Foundation-7 (MCF-7) human breast cancer cell lines were inoculated with NDV VIID and LaSota at concentrations of 10-2, 10-3, 10-4, and 10-5, Paclitaxel (for MCF-7) at concentrations of 0.5, 1, and 2 μM, and Doxorubicin (for Caco-2) at 0.1, 1 and 10 μM in four replicates each. The cytotoxic effect was performed using a neutral red assay for both virus strains and in combination with chemotherapeutic agents. The present study clarified that both VIID and LaSota strains of NDV, particularly at titers of 10-3 and 10-4 TCID50/ml, respectively, displayed a significant (P ⤠0.05) cytotoxic effect on both MCF-7 and Caco-2 cell lines. Moreover, the combined treatment of the TCID50 (Tissue Culture Infective Dose 50) doses of both NDV strains and the tested chemotherapies showed a more significant (P ⤠0.05) cytotoxic effect than the sole use of each. Depending on the results, we can conclude that this study opens the way for further in vivo studies aiming to provide more safe treatment for human cancers, save human lives, and avoid dramatic ends.
Authors and Affiliations
Alaa Abd El-Khalek,Mohamed Khodeir,Amani Saleh,Azza Hassan,Sahar Abdelrahman,
Keywords
Related Articles
- EP ID EP725708
- DOI -
- Views 41
- Downloads 0
Effects of Aqueous and Ethanolic Extracts of Ginger (Zingiber Officinale) Rhizome on Serum Progesterone Level and Markers of Oxidative Stress in African Giant Rat (Cricetomys Gambianus) in Captivity
The African giant rat is a very prolific animal in its natural biotope. But in captivity, this species exhibits poor reproductive performance due to embryonic resorption. These resorptions are due to oxidative stress or...
Isolation of Helicobacter Pylori from Raw Milk and Study on Its Survival in Fermented Milk Products
This research aimed to know if raw milk is a possible source of Helicobacter. pylori infection to human and assessing the inhibitory effect of fermented milk products on H. pylori growth. Sixty samples of cows' milk and...
Nanonephrology In Veterinary Medicine: A Branch Of Nanomedicine For Renal Diseases In Animals
Nanotechnology is a fast-growing technology because of It's capable of solving many problems related to animal production and health and plays important roles in the veterinary medicine field. Nanoparticles are character...
Immuno-Potentiator Effect Of Rapeseed Or Soybean Oil With Foot And Mouth Disease Vaccine In Calves
One of the effective recommendation for control of Foot and Mouth disease virus is the proper vaccination by a highly potent vaccine. Such a vaccine should be specific to the circulated field serotype of foot and mouth d...
Investigation of The Association Between Bovine Viral Diarrhea Virus and Neospora caninum as a Cause of Abortion in Cattle
This study aimed to investigate the complementary association between Bovine Viral Diarrhea Virus (BVDV) and Neospora caninum (NC) in the induction of abortion in cattle. The study was carried out on 116 serum samples co...