The Inhibitory Effect ofCarica papaya cv. Thailand Leaf Extract to the Growth of Enterococcus faecalis In Vitro
Journal Title: Scholars Journal of Dental Sciences - Year 2017, Vol 4, Issue 6
Abstract
Enterecoccusfaecalis is one of resistant bacteria in the medication of root canal treatment. ChKM is mostly used as sterilization agent. Carica papaya leaf extract has been reported to have antibacterial effect from gram-negative bacteria, so could be potentially developed as a root canal sterilization agent. The aim of this study was to determine the inhibitory effect of Thailand varietas of papaya leaf extract to the growth of Enterococcus faecalis. This study was an experimental study with post test only control group design and were tested by diffusion methods with 3 groups concentration of 25%,50%,75% and 2 controls groups:Aquadest as negative control, and ChKM as positive control, each group consisted of 6 samples. The inhibition effects were examined by measure the diameter of the clear zone around the disc. Data were analyzed by One Way ANOVA test and followed by LSD test. Result showed that there were clear zone around the disc, the greater concentration of the extract the greater diameter of the clear zone.Mean of inhibitation zone at concentration of 25%(6,30mm), 50%(7,54mm), 75%(8,36mm), Aquadest (6mm), and ChKM (11,32mm). It had been proved that papaya leaf extract could inhibit the growth of Enterococcus faecalis (p<0,05). The largest diameter of the clear zone was in the concentration of 75%. Thailand varietas of papaya leaf extract could inhibit the growth of Enterococcus faecalis and the most effective inhibitory concentration is 75% but is smaller than positive control (ChKM). Keywords:root canal treatment, antibacterial, Carica papaya cv. Thailand, Enterococcus faecalis
Authors and Affiliations
Yetti Wilda, F. M. Satryani, S. Maemonah ,, Badriyah ,, J. Christyaningsih ,
Keywords
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