THE INTERACTOME: PREDICTING THE PROTEIN-PROTEIN INTERACTIONS IN CELLS
Journal Title: Cellular and Molecular Biology Letters - Year 2009, Vol 14, Issue 1
Abstract
The term Interactome describes the set of all molecular interactions in cells, especially in the context of protein-protein interactions. These interactions are crucial for most cellular processes, so the full representation of the interaction repertoire is needed to understand the cell molecular machinery at the system biology level. In this short review, we compare various methods for predicting protein-protein interactions using sequence and structure information. The ultimate goal of those approaches is to present the complete methodology for the automatic selection of interaction partners using their amino acid sequences and/or three dimensional structures, if known. Apart from a description of each method, details of the software or web interface needed for high throughput prediction on the whole genome scale are also provided. The proposed validation of the theoretical methods using experimental data would be a better assessment of their accuracy.
Authors and Affiliations
Dariusz Plewczyński, Krzysztof Ginalski
Keywords
Related Articles
- EP ID EP83009
- DOI 10.2478/s11658-008-0024-7
- Views 160
- Downloads 0
A MICROARRAY GENE ANALYSIS OF PERIPHERAL WHOLE BLOOD IN NORMAL ADULT MALE RATS AFTER LONG-TERM GH GENE THERAPY
The main aims of this study were to determine the effects of GH gene abuse/misuse in normal animals and to discover genes that could be used as candidate biomarkers for the detection of GH gene therapy abuse/misuse in hu...
LEPTIN REGULATES MMP-2, TIMP-1 AND COLLAGEN SYNTHESIS VIA p38 MAPK IN HL-1 MURINE CARDIOMYOCYTES
A clear association between obesity and heart failure exists and a significant role for leptin, the product of the obese gene, has been suggested. One aspect of myocardial remodeling which characterizes heart failure is...
TRANSCRIPTIONAL PROFILES DURING THE DIFFERENTIATION AND MATURATION OF MONOCYTE-DERIVED DENDRITIC CELLS, ANALYZED USING FOCUSED MICROARRAYS
Dendritic cells (DC) are professional antigen-presenting cells capable of initiating primary immune responses. They have been intensively studied and are used in both basic immunology research and clinical immunotherapy....
A NEW BSMV-BASED VECTOR WITH MODIFIED ß MOLECULE ALLOWS SIMULTANEOUS AND STABLE SILENCING OF TWO GENES
Virus-induced gene silencing is an important tool for functional gene analysis and the vector based on Barley stripe mosaic virus (BSMV) is widely used for the purpose in monocots. Of the tripartite BSMV genome, currentl...
The antioxidant activity of BHT and new phenolic compounds PYA and PPA measuredby chemiluminescence.
The antioxidative properties of two series of new phenolic, amphiphiliccompounds were evaluated using the chemiluminescence (CL) method. 2,2'-Azobis (2-amidinopropane) dihydrochloride(AAPH) was used as a source of free r...