The Isolation of Lactococcus garvieae from Eyes of Diseased Rainbow Trout (Oncorhynchus mykiss) with Exopthalmia
Journal Title: JOURNAL OF ANATOLIAN ENVIRONMENTAL AND ANIMAL SCIENCES - Year 2019, Vol 4, Issue 1
Abstract
In this study, the diseased fish samples were collected from fish farms in the nine different dam lakes during the summer months between July 2014 and September 2018. It is isolated twenty-two bacteria from diseased rainbow trout (Oncorhynchus mykiss) eyes showing exophthalmos symptoms. All isolates identification was carried out by using conventional biochemical tests, API 20 Strep test kit and PCR test. ATCC 43921 reference strain of the Lactococcus garvieae was used as a positive control. The PCR test was confirmed by using the pLG-1 and pLG-2 reference genes (16S rRNA) specific for the L. garvieae species. As a result of this study, all of the twenty-two isolates isolated from the eyes of diseased rainbow trout were confirmed as L. garvieae by PCR test. According to the results of antibiotic susceptibility test, all of the L. garvieae isolates were resistant to streptomycin, sulfamethoxazole, and sulfamethoxazole/trimethoprim. The same isolates have been determined to be resistant to ampicillin (94.2%), oxytetracycline (72.5%), erythromycin (55.07%), oxolinic acid (43.5%), enrofloxacin (26.09%), doxycycline (20.3%), amoxicillin and florfenicol (8,7%) as well. Thus, florfenicol, amoxicillin, doxycycline, and enrofloxacin were found as the most effective antibiotics.
Authors and Affiliations
Fikri Balta, Zeynep Dengiz Balta
Keywords
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- EP ID EP558255
- DOI 10.35229/jaes.527258
- Views 63
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