THE LEVEL OF DIFFERENT FORMS OF HEMOGLOBIN IN THE HEMOLISAT OF THE ERYTHROCYTES OF RATS BLOOD ON CONDITION OF THE LACK OF PROTEIN
Journal Title: Naukovyi visnyk Chernivetskoho universytetu. Biolohiia (Biolohichni systemy) - Year 2014, Vol 6, Issue 2
Abstract
In this work has been researched the general level of hemoglobin and its main derivative - oxyhemoglobin in hemolisates of the rats' erythrocytes under conditions of nutritional deprivation of protein. At the same time considerable attention was paid to the determination of glycosylated hemoglobin content - marker of disruptions of carbohydrate metabolism in the body under conditions of protein deficiency. In order to study the characteristics of these biochemical indicators under conditions of nutritional protein deprivation experimental animals were kept for 28 days on a semi-synthetic low-protein diet on the principle of pair food. Hemolisate of erythrocytes was obtained by Drabkin method with subsequent removal of the reticular stroma. Determination of total hemoglobin was made by a hemoglobincyanide method, oxyhemoglobin - by a spectrophotometric method by means of the molar extinction coefficient at achieving the maximum level of oxygenation of the molecule. The level of glycosylated hemoglobin in red blood cells' hemolisate was determined by reaction with the thiobarbituric aicid. It was found out that under conditions of protein deficiency in rats' blood hemolisate is a decrease in the concentration of total hemoglobin and oxyhemoglobin 1,4 times compared to the control indicators. It should be noted that the decrease of hemoglobin under conditions of protein deficiency on the one hand, it may be due to impaired synthesis of globin polypeptide chains due to a deficiency of aminoacids, on the other hand - a disruption of heme synthesis. At the same time in the experimental group of animals has been observed the decrease of main ligand form of hemoglobin - oxyhemoglobin, which may indicate the disruption of binding and kickback of oxygen and the resulting changes in the affinity of hemoglobin for oxygen. At the same time we studied that hemolisate of red blood cells of rats who were on a low-protein diet has double increase in glycosylated hemoglobin compared to control indicators, which is considered as a prognostic indicator of metabolic hyperglycemia, because it reflacts percentage of hemoglobin, irrevocably conncted to glycose. Glycosylated hemoglobin due to a strong relationship with oxygen gives it hard to tissues, so increasing of its content in the blood causes the occurrence of tissue hypoxia, which is related to insufficient oxygenation of basal membrans.
Authors and Affiliations
Г. П. КОПИЛЬЧУК, І. М. БУЧКОВСЬКА, М. Г. СКРИПНИК
Keywords
Related Articles
- EP ID EP246581
- DOI -
- Views 73
- Downloads 0
ERECHTITES HIERACIFOLIA (L.) RAF. EX DC. (ASTERACEAE) У ЛУЧНИХ КОМПЛЕКСАХ ПОКУТТЯ
Висвітлено еколого-ценотичну приуроченість інвазійного виду північноамериканського походження Erechtites hieracifolia (L.) Raf. ex DC. на території Покуття (урочище «Сіножаті» в околицях с. Старий Гвіздець Коломийського...
Features of adaptation of Schoenus ferrugineus L. To transformed environmental conditions
The factors of anthropogenic transformation of the groups S. ferrugineus have been characterized. According to the floristic classification, investigated communities classified as one class of sedge-moss vegetation in lo...
Активність цитозольних ізоформ ензимів катаболізму ендогенних альдегідів за умов токсичного ураження на фоні білкової недостатності
У роботі визначено активність альдегіддегідрогенази (КФ 1.2.1.3), альдегідредуктази (КФ 1.1.1.21), а також вміст ТБК-активних продуктів і карбонільних похідних протеїнів у цитозольній фракції печінки щурів за умов ацетам...
Історія досліджень рослинного світу регіонального ландшафтного парку «гадяцький» (полтавська область)
У статті наведено результати опрацювання гербарних колекцій та аналізу літературних відомостей щодо дослідження фіторізноманітності природно-заповідної території, яка охороняється у статусі регіонального ландшафтного пар...
Possible protective mechanisms of alpha-ketoglutarate on fruit fly drosophila melanogaster canton s under exposure to different stressors
Alpha-ketoglutaric acid is an important intermediate in Krebs cycle and in metabolism of amino acids. In recent years, the role of alpha-ketoglutaric acid as a powerful natural detoxifying agent was demonstrated. This wo...