The Loop Regions and Substrate Specificity of GH 27 Familiy α-Galactosidases
Journal Title: Scholarly Journal of Food and Nutrition - Year 2018, Vol 1, Issue 1
Abstract
Galacto-oligosaccharides (i.e. stachyose and raffinose) commonly exist in food and feed and are indigestible by human and animals, thereby causing flatulence, gastrointestinal disturbance and low feed efficiency. [1] α-Galactosidases (EC. 3.2.1.22) has hydrolysis ability to degrade these anti-nutritional factors, decrease the viscosity of the diet, reduce the occurrence of diarrhoea, destroy the structure of the cell wall of the plant, promote the nutrition release, improve the utilization efficiency of the nutrients in the feed, increases lean meat rate, enhances immune function and disease resistance of animals. [2,3] α-Galactosidases has great application value in industrial processes of feed, food, and beet sugar production. α-Galactosidases are widely distributed in fungi, bacteria, plants and human (www.cazy.org). Fungal α-galactosidases have maximal activity at pH 3-5, but bacterial α-galactosidases are the optimal pH of 6-7.5.1, [4-6] Some thermostable α-galactosidases have been identified from thermophilic fungi, such as thermomyces lanuginosus, Talaromecys emersonii, and Rhizomucor miehei. [7- 9] Due to high processing temperatures and acidic environment of the gastrointestinal tract, the highly efficient, thermostable and acidicphilic α-galactosidases with broad substrate specificity is of great interest. [10] Base on the sequence similarities, α-galactosidases are divided into Glycoside Hydrolase (GH) families 4, 27, 36, 57, 97, and 110. [11] Most fungal α-galactosidases belong to GH27 and have conserved YLKYDNC catalytic motif and DD(G/C) W binding motif. [12,13] The resolved crystal structures of two GH27 α-galactosidases from Trichoderma reesei (1t0oA) and Saccharomyces cerevisiae (ScAGal, 3LRK) share a (β/α)8 barrel fold and a retaining reaction mechanism. [13,14] Sequence analysis indicated Loops 1, 2, and 4 of α-galactosidase from T. reesei and corresponding loops 1-3 and 6 from ScAGal create new binding sites for formation and breakdown of a covalent glycosyl enzyme intermediate.13,14 In our study, two α-galactosidases Gal27A and Gal27B of family GH27 from the thermophilic Neosartorya fischeri had similar tertiary structures but varied in loop regions and substrate specificity. [15,16] Gal27A had far separate loops and showed higher activity towards raffinose, which was 4.9 - and 3.8- fold for that of melibiose and stachyose.
Authors and Affiliations
Canfang Niu, Peilong Yang’s
Keywords
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- EP ID EP594727
- DOI 10.32474/SJFN.2018.01.000102
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