The Most Frequently Isolated Microorganisms and the Incidence of Positive Culture Report in Patients with Sepsis: A Hospital Based Prospective Study
Journal Title: International Journal of Medical Research Professionals - Year 2017, Vol 3, Issue 4
Abstract
Background: There are many diagnostic tests to rule out the presence or absence of sepsis but blood culture remain the key test for estimating the presence of sepsis. Until recently no biomarker has been able to help differentiate bacterial infection from a viral or non-infectious inflammatory reaction. An ideal marker for bacterial infections should allow an early diagnosis, also inform about the course and prognosis of the disease and facilitate therapeutic decisions. The aim of present study is to establish the most frequently isolated microorganism and the incidence of positive blood culture in patients with sepsis admitted to the hospital. Materials and Methods: The present prospective study was done as a part of research work at Department of Medicine, Dayanand Medical College and Hospital, Ludhiana. Patients admitted in emergency or Intensive Care Unit (ICU) and clinically suspected to have sepsis were included in the study. All the blood and body fluid sample were analyzed for microbiology profile in the Department of Microbiology, Dayanand Medical College and Hospital, Ludhiana. 5- 10 ml of blood was withdrawn and bactec cultures were performed. Smears were prepared from positive blood culture bottles and examined after Gram’s staining. Simultaneously all the positive bottles were sub-cultured on blood Agar and MacConkey’s agar and the plates were then incubated at 37°C for 18-24 hours. Results: In the present study 102 subjects were enrolled out of which 38.2% patients were of sepsis, 42.2% patients were of severe sepsis and 19.6% patients were of septic shock. The most common source of infection in the sepsis and severe sepsis group was lower respiratory infection; 61.54% and 35.00% respectively whereas in the septic shock group UTI (urinary tract infection) was more prevalent (34.88%). The pathogens isolated in blood culture sample are depicted in the table 4. Gram negative pathogens accounted for the majority (87.9%) of positive blood culture. We isolated fungal growth in 3 patients. Conclusion: The most common cause of sepsis was lower respiratory tract infection and the most commonly isolated microorganisms were gram negative bacteria.
Authors and Affiliations
Surinder Pal Singh, Manpreet Kaur Jhinger, Jatinder Pal Singh, Neeraj Singla, Shaminder Kaur, Gurpreet Singh
Keywords
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- EP ID EP546630
- DOI 10.21276/ijmrp.2017.3.4.012
- Views 65
- Downloads 0
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