The new era for diagnostic microbiology laboratories: ‘All things are ready, if our mind be so’
Journal Title: Microbes and Infectious Diseases - Year 2020, Vol 1, Issue 1
Abstract
Identification and characterization of micro-organisms that cause infections are crucial for successful management of patients. For several decades, routine clinical microbiological diagnostic laboratories have been equipped with a growing panel of culture-dependent and culture-independent methods to investigate the microbial etiology of infectious diseases. Considering the disadvantages of traditional methods, multiplex PCR techniques have been routinely endorsed in clinical microbiology laboratories as rapid and sensitive diagnostic and prognostic tool including many PCR panels routinely used in microbial diagnostics. The great advance in medical biotechnology has been associated with development of Matrix-Associated Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI TOF MS). The power of MALDI TOF MS correctly identified 93.2% of organisms to the species level and 5.3% to the genus level. Recently, the application of next-generation sequencing (NGS) technology and its various methodological variants makes it possible to detect different types of microorganisms present within a microbial sample simultaneously, using a culture-independent approach and in a single sequencing run. Over the next 5 to 10 years, although it is unlikely to see NGS completely replacing the conventional culture and susceptibility methods, a wealth of NGS applications will be acquired in the vast majority of diagnostic microbiology laboratories worldwide, providing enhanced diagnostic capabilities and improving the quality of patients care. In Egyptian diagnostic microbiology laboratories, we have to ask ourselves; are we ready to subsist this new era? Sure the answer is: ‘All things are ready, if our mind be so’.
Authors and Affiliations
Ahmed Morad Asaad
Keywords
Related Articles
- EP ID EP683132
- DOI 10.21608/MID.2020.25905.1001
- Views 261
- Downloads 0
Detection of carbapenemase enzymes and genes among carbapenem-resistant Enterobacteriaceae isolates in Suez Canal University Hospitals in Ismailia ,Egypt.
Background: Carbapenem antibiotics are important therapeutic agents in the health care setting, they are frequently used as an empiric therapy for life-threatening infections as well as infections with multi-drug-resista...
The burden of unspecified febrile syndrome in a community hospital of Cuba
Background: The Cuban health-care system recommends the hospital admissions of patients with unspecified febrile syndrome (UFS) to rule out arboviral diseases and to prevent healthcare associated adverse outcomes. Aim: E...
Continuous medical education activities; Case No. 1
A three years old male was presented, fatigued with history of fever for 4 days. He became acutely ill and vomited during lunch. Over the next 4 days he developed fever as high as 40oC that was controlled by Acetaminophe...
The new era for diagnostic microbiology laboratories: ‘All things are ready, if our mind be so’
Identification and characterization of micro-organisms that cause infections are crucial for successful management of patients. For several decades, routine clinical microbiological diagnostic laboratories have been equi...
Evaluation of antibacterial activity of silver nanoparticles against multidrug-resistant Gram negative bacilli clinical isolates from Zagazig University Hospitals
Background: The growing incidence of multidrug resistant (MDR) bacterial infections has become a public health crisis. This work aims to evaluate the in-vitro activity of silver nanoparticles (AgNPs), alone and in combin...