The NH2-Terminal Ig Domains of Insect Projectin could serve as Elastic Elements
Journal Title: Journal of Proteomics and Genomics Research - Year 2012, Vol 1, Issue 1
Abstract
The connecting C-filaments of insect indirect flight muscles have been proposed as one of the elements providing muscle elasticity for the asynchronous muscle physiology of derived insects. Two large modular proteins, kettin/Sallimus and projectin make up these filaments, and for both proteins the N-terminal sequences span the extensible I-band and are proposed as the elastic segments. The C-filaments have not been studied in insects, such as dragonflies, crickets, and Lepidoptera with muscles which are largely synchronous in physiology and display different levels of muscle stiffness. In this paper we focus our efforts on the projectin protein of several insects with synchronous flight muscles; namely dragonfly, cricket, and moth. We provide evidence for the localization of projectin over the sarcomere I-Z-I region that is consistent with the existence of C-filaments in synchronous flight muscles. Additionally, we determine the sequences for the NH2-terminal region of projectin in these insects and describe the presence of alternative splice variants. Using predictors of intrinsically disordered regions, we identify possible unfolded segments, especially around the short linker sequences found between the NH2 Ig domains. We propose a possible picture of projectin NH2-terminal region organized as different segments contributing elastic responses to stretch by either unfolding of highly disordered sequences (PEVK) or reorientation of domains by bending or twisting of disordered linkers between the Ig domains.
Authors and Affiliations
Agnes Ayme-Southgate, Mary Crowe, Richard Southgate
Keywords
Related Articles
- EP ID EP261061
- DOI 10.14302/issn.2326-0793.jpgr-12-112
- Views 135
- Downloads 0
Mechanisms Associated with Acquisition of Resistance to Butyrate-Induced Apoptosis in Colorectal Cancer Cells Using Gene Expression Analysis
Colorectal cancer is one of the most commonly diagnosed cancers worldwide and its prevalence can be reduced by changes to lifestyle and diet. Fermentation of dietary fibre by the gut microbiota and formation of short cha...
GR-RNF43 Regulation in Colorectal Cancer
In contrast to approaches that compare pair-wise control (i.e. normal) to treated (i.e. disease) samples, we compared colorectal cancer samples not only to a set of control samples but also against a wide range of sample...
Rapid and Sensitive High-Performance Liquid Chromatography/Tandem Mass Spectrometry Method for Determination of Danshensu in Rat Plasma
A sensitive, specific and rapid high-performance liquid chromatography/tandem mass spectrometry (hplc–ms/ms) method has been developed and validated for the determination of danshensu (dss) in rat plasma in the present s...
Shotgun Label-Free Proteomic Analyses of the Oyster Parasite Perkinsus Marinus
Perkinsus marinus is an intracellular parasitic protozoan that is responsible for serious disease epizootics in marine bivalve mollusks worldwide. Despite all available information on P. marinus genomics, more baseline d...
Quantitative Proteomics Using 15N SILAC Mouse
In biomedical research the use of mammalian tissues is crucial to increase our understanding of complex human diseases. Mass spectrometry-based proteomic approach has become the most powerful tool of studying large-scale...